Literature DB >> 27345256

Chromosomal localisation of the CD4cre transgene in B6·Cg-Tg(Cd4-cre)1Cwi mice.

Kerstin Westendorf1, Pawel Durek2, Samia Ayew1, Mir-Farzin Mashreghi3, Andreas Radbruch1.   

Abstract

The B6·Cg-Tg(Cd4-cre)1Cwi line expresses CRE recombinase under the control of the promoter and regulatory elements of the Cd4 gene. Here we show that CRE recombinase expression reduces the number and frequencies of CD4 positive subsets in a dose-dependent manner and localize the integration site of the transgenic construct to position 60335693-60341285 (qD) of chromosome 3. The insert contains at least 15 complete sequential copies of the transgenic construct. Based on this information we describe a novel PCR assay for genetic typing of transgenic mice.
Copyright © 2016 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  CD4 cre; CRE recombinase; CRE toxicity; Genotyping; Transgene localisation; Whole genome sequencing

Mesh：

Substances：

Year:  2016        PMID： 27345256     DOI： 10.1016/j.jim.2016.06.005

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  7 in total

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Authors:  Cassandra L Houser; B Paige Lawrence
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Authors:  Chichu Xie; Fangming Zhu; Julie Wang; Weizhou Zhang; Joseph A Bellanti; Bin Li; David Brand; Nancy Olsen; Song Guo Zheng
Journal:  Cells       Date:  2019-10-24       Impact factor: 6.600

7.  The pioneer transcription factors Foxa1 and Foxa2 regulate alternative RNA splicing during thymocyte positive selection.

Authors:  Ching-In Lau; Jasmine Rowell; Diana C Yanez; Anisha Solanki; Susan Ross; Masahiro Ono; Tessa Crompton
Journal:  Development       Date:  2021-07-29       Impact factor: 6.868
  7 in total

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