| Literature DB >> 27342126 |
George Savidis1, William M McDougall1, Paul Meraner1, Jill M Perreira1, Jocelyn M Portmann1, Gaia Trincucci1, Sinu P John2, Aaron M Aker1, Nicholas Renzette1, Douglas R Robbins1, Zhiru Guo3, Sharone Green3, Timothy F Kowalik1, Abraham L Brass4.
Abstract
The flaviviruses dengue virus (DENV) and Zika virus (ZIKV) are severe health threats with rapidly expanding ranges. To identify the host cell dependencies of DENV and ZIKV, we completed orthologous functional genomic screens using RNAi and CRISPR/Cas9 approaches. The screens recovered the ZIKV entry factor AXL as well as multiple host factors involved in endocytosis (RAB5C and RABGEF), heparin sulfation (NDST1 and EXT1), and transmembrane protein processing and maturation, including the endoplasmic reticulum membrane complex (EMC). We find that both flaviviruses require the EMC for their early stages of infection. Together, these studies generate a high-confidence, systems-wide view of human-flavivirus interactions and provide insights into the role of the EMC in flavivirus replication.Entities:
Keywords: AXL; CRISPR/Cas9 screen; EMC; MORR; RNAi screen; Zika virus; arbovirus; dengue virus; flavivirus; yellow fever virus
Mesh：
Year: 2016 PMID： 27342126 DOI： 10.1016/j.celrep.2016.06.028
Source DB: PubMed Journal: Cell Rep Impact factor: 9.423