Mengjie Zhang1, Wang Wei2, Yingming Sun1, Xiu Jiang3, Xiu Ying3, Rui Tao4, Longxing Ni2. 1. Department of Stomatology, The 101 Military Hospital, Wuxi 214000, People's Republic of China. 2. State Key Laboratory of Military Stomatology, Department of Operative Dentistry and Endodontics, School of Stomatology, Fourth Military Medical University, Xi'an 710032, People's Republic of China. 3. School of Stomatology, Anhui Medical University, Anhui 230032, People's Republic of China. 4. Department of Stomatology, The 101 Military Hospital, Wuxi 214000, People's Republic of China. Electronic address: taoruicn@outlook.com.
Abstract
OBJECTIVES: Fish epidermal antimicrobial peptides, such as pleurocidin, are cathelicidins with broad-spectrum antimicrobial activity against gram negative and gram-positive bacteria, as well as fungi. In the current study, we attempted to optimize peptide bioactivity by sequence modification and assess the antimicrobial activities. METHODS: Fifteen pleurocidin analogues were designed, and the efficacy of pleurocidin congeners against common cariogenic microorganisms was tested; furthermore, we performed a preliminary study of the antimicrobial mechanism. We assayed the minimal inhibitory concentration (MIC), minimal bactericide concentration (MBC) and bactericidal kinetics to determine the cell killing activity. Scanning electron microscopy (SEM) was used to observe the bacterial membrane after treatment with congeners' peptides. Human gingival fibroblasts (HGFs) were also used in toxicity studies. RESULTS: The MIC and MBC results indicated that peptide congeners had different antimicrobial activities against the tested oral strains. Toxicity studies indicated that several congener peptides had little effect on human gingival fibroblasts (HGFs) with 5min of in vitro treatment. CONCLUSION: Our findings suggested that several pleurocidin congeners had the antimicrobial effect against Streptococcus mutans, Streptococcus sanguinis and Streptococcus sobrinus.
OBJECTIVES: Fish epidermal antimicrobial peptides, such as pleurocidin, are cathelicidins with broad-spectrum antimicrobial activity against gram negative and gram-positive bacteria, as well as fungi. In the current study, we attempted to optimize peptide bioactivity by sequence modification and assess the antimicrobial activities. METHODS: Fifteen pleurocidin analogues were designed, and the efficacy of pleurocidin congeners against common cariogenic microorganisms was tested; furthermore, we performed a preliminary study of the antimicrobial mechanism. We assayed the minimal inhibitory concentration (MIC), minimal bactericide concentration (MBC) and bactericidal kinetics to determine the cell killing activity. Scanning electron microscopy (SEM) was used to observe the bacterial membrane after treatment with congeners' peptides. Human gingival fibroblasts (HGFs) were also used in toxicity studies. RESULTS: The MIC and MBC results indicated that peptide congeners had different antimicrobial activities against the tested oral strains. Toxicity studies indicated that several congener peptides had little effect on human gingival fibroblasts (HGFs) with 5min of in vitro treatment. CONCLUSION: Our findings suggested that several pleurocidin congeners had the antimicrobial effect against Streptococcus mutans, Streptococcus sanguinis and Streptococcus sobrinus.
Authors: Giorgia Manzo; Charlotte K Hind; Philip M Ferguson; Richard T Amison; Alice C Hodgson-Casson; Katarzyna A Ciazynska; Bethany J Weller; Maria Clarke; Carolyn Lam; Rico C H Man; Blaze G O' Shaughnessy; Melanie Clifford; Tam T Bui; Alex F Drake; R Andrew Atkinson; Jenny K W Lam; Simon C Pitchford; Clive P Page; David A Phoenix; Christian D Lorenz; J Mark Sutton; A James Mason Journal: Commun Biol Date: 2020-11-27