| Literature DB >> 27338712 |
Nathan Broekaert1, Mathias Devreese2, Kristel Demeyere1, Franz Berthiller3, Herbert Michlmayr4, Elisabeth Varga3, Gerhard Adam4, Evelyne Meyer1, Siska Croubels5.
Abstract
The gastrointestinal tract is the first target after ingestion of the mycotoxin deoxynivalenol (DON) via feed and food. Deoxynivalenol is known to affect the proliferation and viability of animal and human intestinal epithelial cells. In addition to DON, feed and food is often co-contaminated with modified forms of DON, such as 3-acetyldeoxynivalenol (3ADON), 15-acetyl-deoxynivalenol (15ADON) and deoxynivalenol-3-β-D-glucoside (DON3G). The goal of this study was to determine the in vitro intrinsic cytotoxicity of these modified forms towards differentiated and proliferative porcine intestinal epithelial cells by means of flow cytometry. Cell death was assessed by dual staining with Annexin-V-fluorescein isothiocyanate (FITC) and propidium iodide (PI), which allows the discrimination of viable (FITC-/PI-), apoptotic (FITC+/PI-) and necrotic cells (FITC+/PI+). Based on the data from the presented pilot in vitro study, it is concluded that cytotoxicity for proliferative cells can be ranked as follows: DON3G ≪ 3ADON < DON≈15ADON.Entities:
Keywords: 15-acetyl-deoxynivalenol (15ADON); 3-acetyl-deoxynivalenol (3ADON); Apoptosis; Deoxynivalenol-3-β-D-glucoside (DON3G); Flow cytometry; IPEC-J2; Necrosis
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Year: 2016 PMID： 27338712 DOI： 10.1016/j.fct.2016.06.012
Source DB: PubMed Journal: Food Chem Toxicol ISSN： 0278-6915 Impact factor: 6.023