Swati S More1, James M Beach2, Robert Vince1. 1. Center for Drug Design Academic Health Center, University of Minnesota, Minneapolis, Minnesota, United States. 2. CytoViva, Inc., Auburn, Alabama, United States.
Abstract
PURPOSE: To describe a spectral imaging system for small animal studies based on noninvasive endoscopy of the retina, and to present time-resolved spectral changes from live Alzheimer's mice prior to cognitive decline, corroborating our previous in vitro findings. METHODS: Topical endoscope fundus imaging was modified to use a machine vision camera and tunable wavelength system for acquiring monochromatic images across the visible to near-infrared spectral range. Alzheimer's APP/PS1 mice and age-matched, wild-type mice were imaged monthly from months 3 through 8 to assess changes in the fundus reflection spectrum. Optical changes were fit to Rayleigh light scatter models as measures of amyloid aggregation. RESULTS: Good quality spectral images of the central retina were obtained. Short-wavelength reflectance from Alzheimer's mice retinae showed significant reduction over time compared to wild-type mice. Optical changes were consistent with an increase in Rayleigh light scattering in neural retina due to soluble Aβ1-42 aggregates. The changes in light scatter showed a monotonic increase in soluble amyloid aggregates over a 6-month period, with significant build up occurring at 7 months. CONCLUSIONS: Hyperspectral imaging technique can be brought inexpensively to the study of retinal changes caused by Alzheimer's disease progression in live small animals. A similar previous finding of reduction in the light reflection over a range of wavelengths in isolated Alzheimer's mice retinae, was reproducible in the living Alzheimer's mice. The technique presented here has a potential for development as an early Alzheimer's retinal diagnostic test in humans, which will support the treatment outcome.
PURPOSE: To describe a spectral imaging system for small animal studies based on noninvasive endoscopy of the retina, and to present time-resolved spectral changes from live Alzheimer'smice prior to cognitive decline, corroborating our previous in vitro findings. METHODS: Topical endoscope fundus imaging was modified to use a machine vision camera and tunable wavelength system for acquiring monochromatic images across the visible to near-infrared spectral range. Alzheimer's APP/PS1mice and age-matched, wild-type mice were imaged monthly from months 3 through 8 to assess changes in the fundus reflection spectrum. Optical changes were fit to Rayleigh light scatter models as measures of amyloid aggregation. RESULTS: Good quality spectral images of the central retina were obtained. Short-wavelength reflectance from Alzheimer'smice retinae showed significant reduction over time compared to wild-type mice. Optical changes were consistent with an increase in Rayleigh light scattering in neural retina due to soluble Aβ1-42 aggregates. The changes in light scatter showed a monotonic increase in soluble amyloid aggregates over a 6-month period, with significant build up occurring at 7 months. CONCLUSIONS: Hyperspectral imaging technique can be brought inexpensively to the study of retinal changes caused by Alzheimer's disease progression in live small animals. A similar previous finding of reduction in the light reflection over a range of wavelengths in isolated Alzheimer'smice retinae, was reproducible in the living Alzheimer'smice. The technique presented here has a potential for development as an early Alzheimer's retinal diagnostic test in humans, which will support the treatment outcome.
Authors: Jeremiah K H Lim; Qiao-Xin Li; Zheng He; Algis J Vingrys; Vickie H Y Wong; Nicolas Currier; Jamie Mullen; Bang V Bui; Christine T O Nguyen Journal: Front Neurosci Date: 2016-11-17 Impact factor: 4.677
Authors: Walter H Moos; Douglas V Faller; Ioannis P Glavas; David N Harpp; Michael H Irwin; Iphigenia Kanara; Carl A Pinkert; Whitney R Powers; Kosta Steliou; Demetrios G Vavvas; Krishna Kodukula Journal: Biores Open Access Date: 2017-12-01
Authors: Ralph N Martins; Victor Villemagne; Hamid R Sohrabi; Pratishtha Chatterjee; Tejal M Shah; Giuseppe Verdile; Paul Fraser; Kevin Taddei; Veer B Gupta; Stephanie R Rainey-Smith; Eugene Hone; Steve Pedrini; Wei Ling Lim; Ian Martins; Shaun Frost; Sunil Gupta; Sid O'Bryant; Alan Rembach; David Ames; Kathryn Ellis; Stephanie J Fuller; Belinda Brown; Samantha L Gardener; Binosha Fernando; Prashant Bharadwaj; Samantha Burnham; Simon M Laws; Anna M Barron; Kathryn Goozee; Eka J Wahjoepramono; Prita R Asih; James D Doecke; Olivier Salvado; Ashley I Bush; Christopher C Rowe; Samuel E Gandy; Colin L Masters Journal: J Alzheimers Dis Date: 2018 Impact factor: 4.472