André Birgy1, Corinne Levy2, Philippe Bidet1, Franck Thollot3, Véronique Derkx4, Stéphane Béchet4, Patricia Mariani-Kurkdjian5, Robert Cohen6, Stéphane Bonacorsi7. 1. IAME, UMR 1137, INSERM, F-75018 Paris, France IAME, UMR 1137, Univ Paris Diderot, Sorbonne Paris Cité, F-75018 Paris, France AP-HP, Laboratoire de Microbiologie, Centre National de Référence associé Escherichia coli, Hôpital Robert-Debré, F-75019 Paris, France. 2. Association Clinique Thérapeutique Infantile du Val de Marne (ACTIV), Saint Maur des Fossés, France Groupe de Pathologie Infectieuse Pédiatrique (GPIP), Paris, France Centre de Recherche Clinique du Centre Hospitalier Intercommunal de Créteil, Créteil, France. 3. Association Clinique Thérapeutique Infantile du Val de Marne (ACTIV), Saint Maur des Fossés, France Association Française de Pédiatrie Ambulatoire (AFPA), Paris, France. 4. Association Clinique Thérapeutique Infantile du Val de Marne (ACTIV), Saint Maur des Fossés, France. 5. AP-HP, Laboratoire de Microbiologie, Centre National de Référence associé Escherichia coli, Hôpital Robert-Debré, F-75019 Paris, France. 6. Association Clinique Thérapeutique Infantile du Val de Marne (ACTIV), Saint Maur des Fossés, France Groupe de Pathologie Infectieuse Pédiatrique (GPIP), Paris, France Centre de Recherche Clinique du Centre Hospitalier Intercommunal de Créteil, Créteil, France Unité Court Séjour, Petits Nourrisson, Service de Néonatologie, Centre Hospitalier Intercommunal de Créteil, Créteil, France. 7. IAME, UMR 1137, INSERM, F-75018 Paris, France IAME, UMR 1137, Univ Paris Diderot, Sorbonne Paris Cité, F-75018 Paris, France AP-HP, Laboratoire de Microbiologie, Centre National de Référence associé Escherichia coli, Hôpital Robert-Debré, F-75019 Paris, France stephane.bonacorsi@aphp.fr.
Abstract
OBJECTIVES: The objective of this study was to evaluate the evolution and risk factors of ESBL-producing Enterobacteriaceae (ESBL-E) carriage in children in the community for a long period distinguishing ST131 and non-ST131 Escherichia coli. PATIENTS AND METHODS: In this prospective study, rectal samples were obtained from children aged 6-24 months by community paediatricians between 2010 and 2015. Demographic characteristics and risk factors for ESBL-E carriage were collected. Distribution of β-lactamase genes, phylogenetic groups, ST131 and virulence factors of resistant E. coli was determined. RESULTS: We enrolled 1886 children; 144 (7.6%) harboured ESBL-E, and this rate increased from 4.8% to 10.2% between 2010 and 2015. Risk factors for ESBL-E carriage were being cared for at home [adjusted OR (aOR) = 1.8, 95% CI = 1.1-2.9], recent antibiotic use (aOR = 1.5, 95% CI = 1.0-2.1) and travel history (aOR = 1.7, 95% CI = 1.1-2.6). Among patients carrying ESBL, E. coli (98%) and CTX-M type (90%) predominated and PapGII adhesin, characteristic of pyelonephritogenic E. coli strains, was rare (7%). In 2015, E. coli isolates frequently belonged to the phylogenetic group B2 (48%), and 37% were ST131 compared with 5% in 2010. Compared with non-ESBL-producing strains, ST131 carriage was associated with hospitalization in the last 6 months (aOR = 3.5, 95% CI = 1.4-8.8). CONCLUSIONS: Between 2010 and 2015, the carriage of ESBL-E in community children doubled because of the massive expansion of the E. coli ST131 clonal group. The risk for carrying ST131 was associated with previous hospitalization, but not, contrary to the counterpart, antibiotic treatment, daycare attendance or travel history.
OBJECTIVES: The objective of this study was to evaluate the evolution and risk factors of ESBL-producing Enterobacteriaceae (ESBL-E) carriage in children in the community for a long period distinguishing ST131 and non-ST131Escherichia coli. PATIENTS AND METHODS: In this prospective study, rectal samples were obtained from children aged 6-24 months by community paediatricians between 2010 and 2015. Demographic characteristics and risk factors for ESBL-E carriage were collected. Distribution of β-lactamase genes, phylogenetic groups, ST131 and virulence factors of resistant E. coli was determined. RESULTS: We enrolled 1886 children; 144 (7.6%) harboured ESBL-E, and this rate increased from 4.8% to 10.2% between 2010 and 2015. Risk factors for ESBL-E carriage were being cared for at home [adjusted OR (aOR) = 1.8, 95% CI = 1.1-2.9], recent antibiotic use (aOR = 1.5, 95% CI = 1.0-2.1) and travel history (aOR = 1.7, 95% CI = 1.1-2.6). Among patients carrying ESBL, E. coli (98%) and CTX-M type (90%) predominated and PapGII adhesin, characteristic of pyelonephritogenic E. coli strains, was rare (7%). In 2015, E. coli isolates frequently belonged to the phylogenetic group B2 (48%), and 37% were ST131 compared with 5% in 2010. Compared with non-ESBL-producing strains, ST131 carriage was associated with hospitalization in the last 6 months (aOR = 3.5, 95% CI = 1.4-8.8). CONCLUSIONS: Between 2010 and 2015, the carriage of ESBL-E in community children doubled because of the massive expansion of the E. coli ST131 clonal group. The risk for carrying ST131 was associated with previous hospitalization, but not, contrary to the counterpart, antibiotic treatment, daycare attendance or travel history.
Authors: Munther S Alnajjar; Mamoon A Aldeyab; Michael G Scott; Mary P Kearney; Glenda Fleming; Fiona Glimore; David Farren; James C McElnay Journal: Infection Date: 2019-05-07 Impact factor: 3.553
Authors: L Surgers; A Boyd; P-Y Boelle; V Lalande; P-A Jolivot; P-M Girard; G Arlet; C Cambier; A Homor; D Decre; J-L Meynard Journal: Eur J Clin Microbiol Infect Dis Date: 2017-02-08 Impact factor: 3.267
Authors: S M S Wilmore; K Kranzer; A Williams; B Makamure; A F Nhidza; J Mayini; T Bandason; J Metcalfe; M P Nicol; I Balakrishnan; M J Ellington; N Woodford; S Hopkins; T D McHugh; R A Ferrand Journal: J Med Microbiol Date: 2017-05-18 Impact factor: 2.472