Masataka Adachi1, Iori Kisu1, Toshihiro Nagai2, Katsura Emoto3, Kouji Banno1, Kiyoko Umene1, Yuya Nogami1, Hideaki Tsuchiya4, Iori Itagaki4,5, Ikuo Kawamoto4, Takahiro Nakagawa4, Kazumasa Ogasawara4,6, Daisuke Aoki1. 1. Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan. 2. Electron Microscope Laboratory, Keio University School of Medicine, Tokyo, Japan. 3. Department of Pathology, Keio University School of Medicine, Tokyo, Japan. 4. Research Center for Animal Life Science, Shiga University of Medical Science, Shiga, Japan. 5. The Corporation for Production and Research of Laboratory Primates, Ibaraki, Japan. 6. Department of Pathology, Division of Pathology and Disease Regulation, Shiga University of Medical Science, Shiga, Japan.
Abstract
INTRODUCTION: The objective of this study was to examine the allowable warm ischemic time and pathological changes due to ischemia and reperfusion injury in the uterus of the cynomolgus monkey as a model for uterus transplantation. MATERIAL AND METHODS: Six female cynomolgus monkeys were used in the study. The uterus was resected from the vaginal canal and connected through the bilateral ovarian and uterine arteries and veins only. One animal was used as a control. In the other five animals, the bilateral uterine and ovarian vessels were clamped for 0.5, 1, 2, 4 and 8 h, respectively. Biopsy of the smooth muscle tissue of corpus uteri was performed after each ischemic time and after subsequent reperfusion for 3 h. Biopsy samples were observed by light and electron microscopy. Menstruation recovery was monitored. RESULTS: There were no particular findings in both light and electron microscopy after ischemia for up to 2 h and after subsequent reperfusion. There were no marked changes after ischemia for 4 h, but dilated nuclear pores and rough endoplasmic reticulum swelling were found after reperfusion. These changes also occurred, along with mitochondrial swelling and cristae loss after ischemia for 8 h, and plasma membrane loss, nuclear fragmentation and chromatin condensation were found after reperfusion. Periodical menstruation restarted in all animals with ischemia up to 4 h, but the animal with ischemia for 8 h had amenorrhea and uterine atrophy. CONCLUSIONS: The uterus of the cynomolgus monkey tolerates warm ischemia for up to 4 h.
INTRODUCTION: The objective of this study was to examine the allowable warm ischemic time and pathological changes due to ischemia and reperfusion injury in the uterus of the cynomolgus monkey as a model for uterus transplantation. MATERIAL AND METHODS: Six female cynomolgus monkeys were used in the study. The uterus was resected from the vaginal canal and connected through the bilateral ovarian and uterine arteries and veins only. One animal was used as a control. In the other five animals, the bilateral uterine and ovarian vessels were clamped for 0.5, 1, 2, 4 and 8 h, respectively. Biopsy of the smooth muscle tissue of corpus uteri was performed after each ischemic time and after subsequent reperfusion for 3 h. Biopsy samples were observed by light and electron microscopy. Menstruation recovery was monitored. RESULTS: There were no particular findings in both light and electron microscopy after ischemia for up to 2 h and after subsequent reperfusion. There were no marked changes after ischemia for 4 h, but dilated nuclear pores and rough endoplasmic reticulum swelling were found after reperfusion. These changes also occurred, along with mitochondrial swelling and cristae loss after ischemia for 8 h, and plasma membrane loss, nuclear fragmentation and chromatin condensation were found after reperfusion. Periodical menstruation restarted in all animals with ischemia up to 4 h, but the animal with ischemia for 8 h had amenorrhea and uterine atrophy. CONCLUSIONS: The uterus of the cynomolgus monkey tolerates warm ischemia for up to 4 h.
Authors: Francisco Miguel Sánchez-Margallo; Belén Moreno-Naranjo; María Del Mar Pérez-López; Elena Abellán; José Antonio Domínguez-Arroyo; José Mijares; Ignacio Santiago Álvarez Journal: Sci Rep Date: 2019-05-30 Impact factor: 4.379
Authors: Katarzyna J Szymanska; Menekse Göker; Melissa Bol; Jo Van Dorpe; Steven Weyers; Luc Leybaert Journal: PLoS One Date: 2020-12-10 Impact factor: 3.240