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Literature DB >> 273249

Egg surface glycoprotein receptor for sea urchin sperm bindin.

Abstract

Bindin is an insoluble protein coating the sperm acrosome process and mediating the adhesion of sperm to sea urchin eggs. Milligrams of bindin have been isolated. Here we report the identification, isolation, and partial characterization of a high molecular weight, trypsin-sensitive glycoprotein fraction from the sea urchin egg surface having species-specific affinity for bindin. This glycoprotein may be the egg surface receptor for bindin. The bindin receptor was released from 125-I-labeled eggs by parthenogenetic activation of eggs with ionophore A23187 in the presence of soybean trypsin inhibitor. The receptor has an isoelectric point of 4.02 and a molecular weight in sea water greater than or equal to 5 X 10(6), suggesting that it is an aggregate. It contains 34% neutral sugars, which are galactose and mannose.

Entities: Chemical Species

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Year: 1978 PMID： 273249 PMCID： PMC411361 DOI： 10.1073/pnas.75.2.881

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

18 in total

6. Extracellular coats on the surface of Strongylocentrotus purpuratus eggs: stereo electron microscopy of quick-frozen and deep-etched specimens.

Authors: D E Chandler; C J Kazilek
Journal: Cell Tissue Res Date: 1986 Impact factor: 5.249

10. Signal transduction of fertilization in frog eggs and anti-apoptotic mechanism in human cancer cells: common and specific functions of membrane microdomains.

Authors: Ken-Ichi Sato
Journal: Open Biochem J Date: 2008-04-29

Egg surface glycoprotein receptor for sea urchin sperm bindin.

1. A study of factors involved in induction of the acrosomal reaction in sperm of the sea urchin, Arbacia punctulata.

2. The role of calcium ions in the acrosome reaction of sea urchin sperm: regulation of exocytosis.

3. Protein measurement with the Folin phenol reagent.

4. Isolation of bindin: the protein responsible for adhesion of sperm to sea urchin eggs.

5. Heat sensitivity of the cortical granule protease from hamster eggs.

6. Methods for quantitating sea urchin sperm-egg binding.

7. Method for determination of the sulfate content of glycosaminoglycans.

8. Species specific agglutination of eggs by bindin isolated from sea urchin sperm.

9. Identification of a sperm receptor on the surface of the eggs of the sea urchin Arbacia punctulata.

10. Isolation and characterization of the vitelline layer of sea urchin eggs.

Review 1. On the possible role of endogenous lectins in early animal development.

Review 2. [Molecular biology of gamete conjugation].

3. Ultrastructural and experimental investigations of sperm-egg interactions in fertilization ofHydra carnea.

Review 4. Sulfated glycans in sea urchin fertilization.

5. Bufo arenarum egg jelly coat: purification and characterization of two highly glycosylated proteins.

6. Extracellular coats on the surface of Strongylocentrotus purpuratus eggs: stereo electron microscopy of quick-frozen and deep-etched specimens.

Review 7. Density-dependent regulation of cell growth: an example of a cell-cell recognition phenomenon.

8. The species-specific egg receptor for sea urchin sperm adhesion is EBR1,a novel ADAMTS protein.

9. Species-specific inhibition of fertilization by a peptide derived from the sperm protein bindin.

10. Signal transduction of fertilization in frog eggs and anti-apoptotic mechanism in human cancer cells: common and specific functions of membrane microdomains.