Literature DB >> 27320049

CRISPR/Cas9: A New Tool for RNA Imaging in Live Cells.

Kristina Rau1, Andrea Rentmeister2.   

Abstract

Cas9 can be implemented as an RNA-targeting system to track mRNA in living cells. Nuclear export is enabled by efficient targeting of GFP-fused Cas9 to an endogenous mRNA. The approach provides a new and versatile platform for RNA-targeting with applications in RNA imaging and beyond.
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords:  CRISPR; Cas9; RNA; RNA imaging; RNA tracking; fluorescent probes

Mesh：

Substances：

Year:  2016        PMID： 27320049     DOI： 10.1002/cbic.201600342

Source DB:  PubMed          Journal:  Chembiochem        ISSN： 1439-4227            Impact factor:   3.164


  4 in total

Review 1.  New variants of CRISPR RNA-guided genome editing enzymes.

Authors:  Jana Murovec; Žan Pirc; Bing Yang
Journal:  Plant Biotechnol J       Date:  2017-05-09       Impact factor: 9.803

2.  Live cell imaging and proteomic profiling of endogenous NEAT1 lncRNA by CRISPR/Cas9-mediated knock-in.

Authors:  Bohong Chen; Shengcheng Deng; Tianyu Ge; Miaoman Ye; Jianping Yu; Song Lin; Wenbin Ma; Zhou Songyang
Journal:  Protein Cell       Date:  2020-05-26       Impact factor: 14.870

3.  Sequence-specific m6A demethylation in RNA by FTO fused to RCas9.

Authors:  Kristina Rau; Lukas Rösner; Andrea Rentmeister
Journal:  RNA       Date:  2019-07-01       Impact factor: 4.942

Review 4.  Class 2 CRISPR/Cas: an expanding biotechnology toolbox for and beyond genome editing.

Authors:  Yuyi Tang; Yan Fu
Journal:  Cell Biosci       Date:  2018-11-12       Impact factor: 7.133
  4 in total

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