Literature DB >> 27317301

Dynamic Imaging Reveals Coordinate Effects of Cyclic Stretch and Substrate Stiffness on Endothelial Integrity.

Arkaprava Dan1, Ryan B Huang1, Deborah E Leckband2,3.   

Abstract

We describe an equibiaxial cell stretcher and hybrid, elastic membrane platform designed for dynamic imaging of cells on substrates with physiological stiffness undergoing cyclic stretch. Studies enabled by this device revealed that both substrate stiffness and cyclic stretch coordinately protect pulmonary endothelial monolayers against thrombin-induced disruption. The fluorescence imaging possible with the designed hybrid membranes further revealed similarities and differences in actin and cell dynamics during monolayer recovery. The improved live-cell imaging capabilities of this platform, when used in conjunction with fluorescent probes, will have broad applications for investigations of the impact of biochemical stimuli and mechanotransduction mechanisms on mechanically perturbed tissues.

Entities:  

Keywords:  Cell stretcher; Dynamic fluorescence imaging; Endothelial mechanics; Mechanotransduction

Mesh：

Substances：

Year:  2016        PMID： 27317301      PMCID： PMC5114158          DOI： 10.1007/s10439-016-1677-4

Source DB:  PubMed          Journal:  Ann Biomed Eng        ISSN： 0090-6964            Impact factor:   3.934


  27 in total

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2.  Substrate stiffening promotes endothelial monolayer disruption through enhanced physical forces.

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3.  Differential regulation of pulmonary endothelial monolayer integrity by varying degrees of cyclic stretch.

Authors:  Anna A Birukova; Santipongse Chatchavalvanich; Alexander Rios; Kamon Kawkitinarong; Joe G N Garcia; Konstantin G Birukov
Journal:  Am J Pathol       Date:  2006-05       Impact factor: 4.307

4.  Cell-ECM traction force modulates endogenous tension at cell-cell contacts.

Authors:  Venkat Maruthamuthu; Benedikt Sabass; Ulrich S Schwarz; Margaret L Gardel
Journal:  Proc Natl Acad Sci U S A       Date:  2011-03-07       Impact factor: 11.205

5.  Device for the application of a dynamic biaxially uniform and isotropic strain to a flexible cell culture membrane.

Authors:  J L Schaffer; M Rizen; G J L'Italien; A Benbrahim; J Megerman; L C Gerstenfeld; M L Gray
Journal:  J Orthop Res       Date:  1994-09       Impact factor: 3.494

Review 6.  Mechanotransduction in vascular physiology and atherogenesis.

Authors:  Cornelia Hahn; Martin A Schwartz
Journal:  Nat Rev Mol Cell Biol       Date:  2009-01       Impact factor: 94.444

Review 7.  The genetic basis for altered blood vessel function in disease: large artery stiffening.

Authors:  Alex Agrotis
Journal:  Vasc Health Risk Manag       Date:  2005

8.  Hydraulic fracture during epithelial stretching.

Authors:  Laura Casares; Romaric Vincent; Dobryna Zalvidea; Noelia Campillo; Daniel Navajas; Marino Arroyo; Xavier Trepat
Journal:  Nat Mater       Date:  2015-02-09       Impact factor: 43.841

9.  Development of polydimethylsiloxane substrates with tunable elastic modulus to study cell mechanobiology in muscle and nerve.

Authors:  Rachelle N Palchesko; Ling Zhang; Yan Sun; Adam W Feinberg
Journal:  PLoS One       Date:  2012-12-11       Impact factor: 3.240

10.  Serine/threonine kinase-protein kinase B and extracellular signal-regulated kinase regulate ventilator-induced pulmonary fibrosis after bleomycin-induced acute lung injury: a prospective, controlled animal experiment.

Authors:  Li-Fu Li; Shuen-Kuei Liao; Chung-Chi Huang; Ming-Jui Hung; Deborah A Quinn
Journal:  Crit Care       Date:  2008-08-09       Impact factor: 9.097
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  4 in total

1.  Substrate stiffness and VE-cadherin mechano-transduction coordinate to regulate endothelial monolayer integrity.

Authors:  Roberto C Andresen Eguiluz; Kerim B Kaylan; Gregory H Underhill; Deborah E Leckband
Journal:  Biomaterials       Date:  2017-06-09       Impact factor: 12.479

2.  Mechanical disruption of E-cadherin complexes with epidermal growth factor receptor actuates growth factor-dependent signaling.

Authors:  Brendan Sullivan; Taylor Light; Vinh Vu; Adrian Kapustka; Kalina Hristova; Deborah Leckband
Journal:  Proc Natl Acad Sci U S A       Date:  2022-01-25       Impact factor: 12.779

Review 3.  Integration of substrate- and flow-derived stresses in endothelial cell mechanobiology.

Authors:  Claire A Dessalles; Claire Leclech; Alessia Castagnino; Abdul I Barakat
Journal:  Commun Biol       Date:  2021-06-21

4.  In Vitro Model of Stretch-Induced Lung Injury to Study Different Lung Ventilation Regimens and the Role of Sedatives.

Authors:  Yusuke Mitsui; Sophia Koutsogiannaki; Miho Fujiogi; Koichi Yuki
Journal:  Transl Perioper Pain Med       Date:  2020-05-15
  4 in total

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