Literature DB >> 27316991

Application of Fluorescence Polarization in HTS Assays.

Xinyi Huang1, Ann Aulabaugh2.   

Abstract

Steady-state measurements of fluorescence polarization have been widely adopted in the field of high-throughput screening for the study of biomolecular interactions. This chapter reviews the basic theory of fluorescence polarization, the underlying principle for using fluorescence polarization to study interactions between small-molecule fluorophores and macromolecular targets, and representative applications of fluorescence polarization in high-throughput screening.

Keywords:  Anisotropy; Competition binding; FP; High-throughput screening; Polarization

Mesh：

Substances：

Year:  2016        PMID： 27316991     DOI： 10.1007/978-1-4939-3673-1_7

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  7 in total

1.  Targeting S100 Calcium-Binding Proteins with Small Molecule Inhibitors.

Authors:  Paul T Wilder; Kristen M Varney; David J Weber
Journal:  Methods Mol Biol       Date:  2019

2.  POLArIS, a versatile probe for molecular orientation, revealed actin filaments associated with microtubule asters in early embryos.

Authors:  Ayana Sugizaki; Keisuke Sato; Kazuyoshi Chiba; Kenta Saito; Masahiko Kawagishi; Yuri Tomabechi; Shalin B Mehta; Hirokazu Ishii; Naoki Sakai; Mikako Shirouzu; Tomomi Tani; Sumio Terada
Journal:  Proc Natl Acad Sci U S A       Date:  2021-03-16       Impact factor: 12.779

3.  Rational design of a sensitivity-enhanced tracer for discovering efficient APC-Asef inhibitors.

Authors:  Jie Zhong; Yuegui Guo; Shaoyong Lu; Kun Song; Ying Wang; Li Feng; Zhen Zheng; Qiufen Zhang; Jiacheng Wei; Peng Sang; Yan Shi; Jianfeng Cai; Guoqiang Chen; Chen-Ying Liu; Xiuyan Yang; Jian Zhang
Journal:  Nat Commun       Date:  2022-08-24       Impact factor: 17.694

Review 4.  Intrinsically Fluorescent Anti-Cancer Drugs.

Authors:  Md Lutful Kabir; Feng Wang; Andrew H A Clayton
Journal:  Biology (Basel)       Date:  2022-07-28

5.  Creation of Fluorescent RXR Antagonists Based on CBTF-EE and Application to a Fluorescence Polarization Binding Assay.

Authors:  Maho Takioku; Yuta Takamura; Michiko Fujihara; Masaki Watanabe; Shoya Yamada; Mayu Kawasaki; Sohei Ito; Shogo Nakano; Hiroki Kakuta
Journal:  ACS Med Chem Lett       Date:  2021-05-07       Impact factor: 4.632

Review 6.  Epigenetic assays for chemical biology and drug discovery.

Authors:  Sheraz Gul
Journal:  Clin Epigenetics       Date:  2017-04-21       Impact factor: 6.551

Review 7.  Assay Systems for Profiling Deubiquitinating Activity.

Authors:  Jinhong Cho; Jinyoung Park; Eunice EunKyeong Kim; Eun Joo Song
Journal:  Int J Mol Sci       Date:  2020-08-06       Impact factor: 5.923
  7 in total

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