Fatma Zohra Mellouk1,2, Sofiane Bakour1, Sameh Meradji2, Charbel Al-Bayssari1, Mohamed Cherif Bentakouk3, Fatiha Zouyed4, Abdelghani Djahoudi5, Nafissa Boutefnouchet2, Jean Marc Rolain1. 1. 1 Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UM 63, CNRS 7278, IRD 198, INSERM 1095, IHU Méditerranée Infection, Faculté de Médecine et de Pharmacie, Aix-Marseille-Université , Marseille, France . 2. 2 Laboratoire de Biochimie et de Microbiologie Appliquée, Département de Biochimie, Université Badji Mokhtar-Annaba , Annaba, Algérie. 3. 3 Laboratoire de Réanimation Centre et le Traitement Burns , Annaba, Algérie. 4. 4 Laboratoire central d'EPH EL Hadjar , Annaba, Algérie. 5. 5 Département de Pharmacie, Université Badji Mokhtar-Annaba , Annaba, Algérie.
Abstract
PURPOSE: The aim of the study was to investigate the prevalence and molecular support of carbapenem resistance in gram-negative bacilli clinical isolates collected between March 2013 and March 2015 in three cities (Annaba, Skikda, and Guelma) in northeastern Algeria. RESULTS: One hundred eighty-six isolates were identified as Enterobacteriaceae (161), Pseudomonas aeruginosa (18), and Acinetobacter baumannii (7). Thirty-six of 186 (19.3%) were resistant to carbapenems. Among them, 11 harbored carbapenemase genes, including blaOXA-48 (2 Klebsiella pneumoniae), blaVIM-4 (2 P. aeruginosa), blaNDM-1 (2 A. baumannii), and blaOXA-23 (5 A. baumannii). In addition, other β-lactamases were detected: blaCTX-M-(15/66/139), blaSHV-(28/85/1/133), and blaTEM-1. All imipenem-resistant P. aeruginosa displayed OprD mutations. Multilocus sequence typing demonstrated the presence of ST 404 and ST 219 in K. pneumoniae, ST 2 and ST 85 in A. baumannii, and ST (244, 1076, 241, 227, and 233) in P. aeruginosa. CONCLUSION: In this study, we report the first detection of P. aeruginosa ST 1076 harboring the blaVIM-4 gene in African countries in two cities (Annaba and Skikda) in northeastern Algeria. Additionally, we report the first detection of blaOXA-48 in K. pneumoniae ST 404 and ST 219 in Algerian cities (Annaba and Skikda).
PURPOSE: The aim of the study was to investigate the prevalence and molecular support of carbapenem resistance in gram-negative bacilli clinical isolates collected between March 2013 and March 2015 in three cities (Annaba, Skikda, and Guelma) in northeastern Algeria. RESULTS: One hundred eighty-six isolates were identified as Enterobacteriaceae (161), Pseudomonas aeruginosa (18), and Acinetobacter baumannii (7). Thirty-six of 186 (19.3%) were resistant to carbapenems. Among them, 11 harbored carbapenemase genes, including blaOXA-48 (2 Klebsiella pneumoniae), blaVIM-4 (2 P. aeruginosa), blaNDM-1 (2 A. baumannii), and blaOXA-23 (5 A. baumannii). In addition, other β-lactamases were detected: blaCTX-M-(15/66/139), blaSHV-(28/85/1/133), and blaTEM-1. All imipenem-resistant P. aeruginosa displayed OprD mutations. Multilocus sequence typing demonstrated the presence of ST 404 and ST 219 in K. pneumoniae, ST 2 and ST 85 in A. baumannii, and ST (244, 1076, 241, 227, and 233) in P. aeruginosa. CONCLUSION: In this study, we report the first detection of P. aeruginosa ST 1076 harboring the blaVIM-4 gene in African countries in two cities (Annaba and Skikda) in northeastern Algeria. Additionally, we report the first detection of blaOXA-48 in K. pneumoniae ST 404 and ST 219 in Algerian cities (Annaba and Skikda).
Authors: Rym Lalaoui; Ana Djukovic; Sofiane Bakour; Linda Hadjadj; Jaime Sanz; Miguel Salavert; Jose Luis López-Hontangas; Miguel A Sanz; Carles Ubeda; Jean-Marc Rolain Journal: Antimicrob Resist Infect Control Date: 2019-10-29 Impact factor: 4.887