Moti Moskovitz1, Maya Dotan2, Uri Zilberman3. 1. Department of Pediatric Dentistry, The Hebrew University - Hadassah School of Dental Medicine, P.O.Box 12272, 9112102, Jerusalem, Israel. motim@md.huji.ac.il. 2. Department of Pediatric Dentistry, The Hebrew University - Hadassah School of Dental Medicine, P.O.Box 12272, 9112102, Jerusalem, Israel. 3. Pediatric Dental Unit, Barzilai Medical Center, Ashkelon, Israel.
Abstract
OBJECTIVES: The present study explored the histological and chemical effects of infantile thiamine deficiency (ITD) on enamel development through the examination of exfoliated deciduous teeth from a patient who had been fed during his first year of life with a thiamine-deficient milk substitute. MATERIALS AND METHODS: Ground sections derived from six exfoliated primary teeth were examined. Slices from a light microscope were photographed for histological analysis. We calculated the time when the amelogenesis insults occurred, and the data were cross-examined with the patient's medical history. We then measured the enamel content of calcium, phosphate, oxygen, carbon, and magnesium on two lines from the dentino-enamel junction (DEJ) to the outer surface using an energy dispersive X-ray spectrometer. RESULTS: Carbon (organic matter) concentration in postnatal enamel was 2.37 times higher in ITD, phosphate levels were lower, and magnesium and calcium levels tended to be higher in ITD teeth. CONCLUSION: Chemical and histological analysis enabled us to confirm that thiamine deficiency in infancy impaired postnatal amelogenesis and resulted in less calcified enamel with a higher level of organic matter. Higher postnatal enamel carbon and magnesium concentration found in ITD may derive from either impaired mineralization caused by disturbed cellular metabolism or indirect damage to the ameloblasts due to the physical condition. Ca/P mean ratio in ITD teeth was higher than the mean ratio in the control displaying a damaged mineralization process. CLINICAL RELEVANCE: This is probably the first description of infantile thiamine deficiency effect on amelogenesis resulting in less calcified enamel.
OBJECTIVES: The present study explored the histological and chemical effects of infantile thiamine deficiency (ITD) on enamel development through the examination of exfoliated deciduous teeth from a patient who had been fed during his first year of life with a thiamine-deficient milk substitute. MATERIALS AND METHODS: Ground sections derived from six exfoliated primary teeth were examined. Slices from a light microscope were photographed for histological analysis. We calculated the time when the amelogenesis insults occurred, and the data were cross-examined with the patient's medical history. We then measured the enamel content of calcium, phosphate, oxygen, carbon, and magnesium on two lines from the dentino-enamel junction (DEJ) to the outer surface using an energy dispersive X-ray spectrometer. RESULTS:Carbon (organic matter) concentration in postnatal enamel was 2.37 times higher in ITD, phosphate levels were lower, and magnesium and calcium levels tended to be higher in ITD teeth. CONCLUSION: Chemical and histological analysis enabled us to confirm that thiaminedeficiency in infancy impaired postnatal amelogenesis and resulted in less calcified enamel with a higher level of organic matter. Higher postnatal enamel carbon and magnesium concentration found in ITD may derive from either impaired mineralization caused by disturbed cellular metabolism or indirect damage to the ameloblasts due to the physical condition. Ca/P mean ratio in ITD teeth was higher than the mean ratio in the control displaying a damaged mineralization process. CLINICAL RELEVANCE: This is probably the first description of infantile thiamine deficiency effect on amelogenesis resulting in less calcified enamel.
Entities:
Keywords:
Ameloblasts; Beriberi; Infantile thiamine deficiency; Striae of Retzius