Mehri Ghafourian1, Neda Ganjalikhanhakemi2, Ali Asghar Hemmati3, Rouhullah Dehghani4, Wesam Kooti5. 1. Health Research Institute, Research Center of Thalassemia and Hemoglobinopathy, Department of Immunology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. 2. Department of Pharmacology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. 3. Department of Pharmacology and Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. 4. Department of Environmental Health, School of Health and Social Determinants of Health (SDH), Research Center, Kashan University of Medical Sciences, Kashan, Iran. 5. Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Abstract
BACKGROUND: The aim of this study was to investigate the effect of Hemiscorpius lepturus venom on leukocytes and the leukocyte subgroups in peripheral blood of rat. METHODS: In this experimental study, sixty N-Mari rats were divided into three groups of 20 rats. Then the rats in each group were divided into four subgroups based on the blood sampling time that was 2, 6, 24 and 48 hours after the venom injection, respectively. The control group did not receive anything, however, the first and the second experimental groups received 0.1 and 0.01mg/kg of venom, subcutaneously. In accordance with a designated four sampling times, the blood sampling was carried out in three groups. After RBC lysis, the leukocytes and leukocyte sub-populations were determined and counted using appropriate hematological standard methods. RESULTS: The leukocyte and the neutrophil count at two (P<0.05), six (P<0.01) and 24 (P<0.05) hours after the venom injection showed a significant decline compared with the control group, this decrease was significant at the dose of 0.1 mg/kg until 48 hours after the venom injection (P<0.05). The lymphocyte count showed a significant decline throughout the all hours of the experiment, compared with the control group (P<0.05). CONCLUSION: Leukocytes are probably affected by the cytotoxicity effect of the H. lepturus venom in a dose-dependent manner. This could be a wakeup call for the medical staff to perform quick and accurate treatment in the least time possible.
BACKGROUND: The aim of this study was to investigate the effect of Hemiscorpius lepturus venom on leukocytes and the leukocyte subgroups in peripheral blood of rat. METHODS: In this experimental study, sixty N-Mari rats were divided into three groups of 20 rats. Then the rats in each group were divided into four subgroups based on the blood sampling time that was 2, 6, 24 and 48 hours after the venom injection, respectively. The control group did not receive anything, however, the first and the second experimental groups received 0.1 and 0.01mg/kg of venom, subcutaneously. In accordance with a designated four sampling times, the blood sampling was carried out in three groups. After RBC lysis, the leukocytes and leukocyte sub-populations were determined and counted using appropriate hematological standard methods. RESULTS: The leukocyte and the neutrophil count at two (P<0.05), six (P<0.01) and 24 (P<0.05) hours after the venom injection showed a significant decline compared with the control group, this decrease was significant at the dose of 0.1 mg/kg until 48 hours after the venom injection (P<0.05). The lymphocyte count showed a significant decline throughout the all hours of the experiment, compared with the control group (P<0.05). CONCLUSION: Leukocytes are probably affected by the cytotoxicity effect of the H. lepturus venom in a dose-dependent manner. This could be a wakeup call for the medical staff to perform quick and accurate treatment in the least time possible.
Authors: Amir Jalali; Mohammad H Pipelzadeh; Mohammad Taraz; Ali Khodadadi; Manocher Makvandi; Edward G Rowan Journal: Eur Cytokine Netw Date: 2011-03 Impact factor: 2.737
Authors: Alfredo Celis; Ramón Gaxiola-Robles; Elizabeth Sevilla-Godínez; María de Jesús Orozco Valerio; Jesús Armas Journal: Rev Panam Salud Publica Date: 2007-06
Authors: Mohammad H Pipelzadeh; Amir Jalali; Mohammad Taraz; Roya Pourabbas; Abbas Zaremirakabadi Journal: Toxicon Date: 2007-08-06 Impact factor: 3.033