Elisabeth Botelho-Nevers1, Florence Grattard2, Alain Viallon3, Séverine Allegra4, Sophie Jarraud5, Paul Verhoeven2, Adrien Marcuccilli3, Fréderic Lucht1, Bruno Pozzetto2, Philippe Berthelot6. 1. GIMAP EA 3064 (Groupe Immunité des Muqueuses et Agents Pathogènes), COMUE of Lyon, 42023 Saint-Etienne, France; Infectious Diseases Department, University Hospital of Saint-Etienne, 42055 Saint-Etienne Cedex 02, France. 2. GIMAP EA 3064 (Groupe Immunité des Muqueuses et Agents Pathogènes), COMUE of Lyon, 42023 Saint-Etienne, France; Laboratory of Infectious Agents and Hygiene, University Hospital of Saint-Etienne, 42055 Saint-Etienne Cedex 02, France. 3. Emergency Unit, University Hospital of Saint-Etienne, 42055 Saint-Etienne Cedex 02, France. 4. GIMAP EA 3064 (Groupe Immunité des Muqueuses et Agents Pathogènes), COMUE of Lyon, 42023 Saint-Etienne, France. 5. Reference National Centre for Legionella, Centre de Biologie et Pathologie Est, Hospices Civils de Lyon, Lyon, France. 6. GIMAP EA 3064 (Groupe Immunité des Muqueuses et Agents Pathogènes), COMUE of Lyon, 42023 Saint-Etienne, France; Infectious Diseases Department, University Hospital of Saint-Etienne, 42055 Saint-Etienne Cedex 02, France; Laboratory of Infectious Agents and Hygiene, University Hospital of Saint-Etienne, 42055 Saint-Etienne Cedex 02, France. Electronic address: philippe.berthelot@chu-st-etienne.fr.
Abstract
OBJECTIVES: Legionnaires' disease (LD) is a severe disease associated with community and hospital-acquired pneumonia, frequently under diagnosed. The main aim of our study was to determine the value of PCR for the diagnosis of LD in routine clinical practice. METHODS: In a prospective study, from March 2007 to April 2010, the value of PCR on non-invasive respiratory specimens (NIRS) was compared to those of the other available tools for LD diagnosis in patients hospitalized for pneumonia. RESULTS: Among 254 consecutive cases of pneumonia included, 24 cases were LD (19 confirmed and 5 probable) representing the first documented microbiological etiology. Molecular diagnosis of LD was performed on NIRS by using 16S rRNA PCR, and secondarily mip PCR, with no discrepant results between the 2 methods: it was found positive in 14 cases and led to identify 2 supplementary probable cases of LD. Based on clinical and at least 2 positive LD tests, PCR yielded a better diagnostic value than antigen urinary test (12 vs 10 cases). CONCLUSION: These results revealed that molecular diagnosis of LD on NIRS is reliable and may contribute to better identify cases of LD.
OBJECTIVES:Legionnaires' disease (LD) is a severe disease associated with community and hospital-acquired pneumonia, frequently under diagnosed. The main aim of our study was to determine the value of PCR for the diagnosis of LD in routine clinical practice. METHODS: In a prospective study, from March 2007 to April 2010, the value of PCR on non-invasive respiratory specimens (NIRS) was compared to those of the other available tools for LD diagnosis in patients hospitalized for pneumonia. RESULTS: Among 254 consecutive cases of pneumonia included, 24 cases were LD (19 confirmed and 5 probable) representing the first documented microbiological etiology. Molecular diagnosis of LD was performed on NIRS by using 16S rRNA PCR, and secondarily mip PCR, with no discrepant results between the 2 methods: it was found positive in 14 cases and led to identify 2 supplementary probable cases of LD. Based on clinical and at least 2 positive LD tests, PCR yielded a better diagnostic value than antigen urinary test (12 vs 10 cases). CONCLUSION: These results revealed that molecular diagnosis of LD on NIRS is reliable and may contribute to better identify cases of LD.
Authors: Hila Mizrahi; Avi Peretz; René Lesnik; Yana Aizenberg-Gershtein; Sara Rodríguez-Martínez; Yehonatan Sharaby; Nina Pastukh; Ingrid Brettar; Manfred G Höfle; Malka Halpern Journal: Sci Rep Date: 2017-01-06 Impact factor: 4.379