H Ghaedi1, M Tabasinezhad2, B Alipoor3, F Shokri1, A Movafagh1, R Mirfakhraie1, M D Omrani4, A Masotti5. 1. Medical Genetics Department, School of Medicine, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 2. Medical Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran. 3. Clinical Biochemistry Department, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. 4. Medical Genetics Department, School of Medicine, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. davood_omrani@yahoo.co.uk. 5. Gene Expression - Microarrays Laboratory, Bambino Gesù Children's Hospital-IRCCS, Viale di San Paolo 15, 00146, Rome, Italy. andrea.masotti@opbg.net.
Abstract
PURPOSE: The study was aimed at investigating the association between hsa-mir-27a polymorphism rs895819 (T/C) and type 2 diabetes mellitus (T2DM) susceptibility in a large Iranian cohort. METHODS: In this case-control study, the investigated population consisted of T2DM patients (n = 204) and sex- and age-matched controls (n = 209). We used the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) for genotyping. RESULTS: We observed significant differences between T2DM patients and controls for weight (p = 0.002), BMI (p < 0.001), systolic blood pressure (p < 0.001), diastolic blood pressure (p < 0.001), fasting plasma glucose (p < 0.001), triglyceride (p = 0.004) and LDL cholesterol (p = 0.051). Moreover, we found that genotype distributions were significantly different between groups (p < 0.05) and that the rs895819-C allele is more frequent in controls (p = 0.030, OR = 0.72, 95 % CI 0.53-0.97). CONCLUSION: Our study shows that rs895819 in hsa-mir-27a is associated with T2DM susceptibility and that the C allele conveyed a protective role against T2DM. Larger multicentric and specific functional studies will be necessary to obtain a deeper comprehension of the role of rs895819 and hsa-mir-27a and how they are involved in the development of diabetes.
PURPOSE: The study was aimed at investigating the association between hsa-mir-27a polymorphism rs895819 (T/C) and type 2 diabetes mellitus (T2DM) susceptibility in a large Iranian cohort. METHODS: In this case-control study, the investigated population consisted of T2DM patients (n = 204) and sex- and age-matched controls (n = 209). We used the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) for genotyping. RESULTS: We observed significant differences between T2DM patients and controls for weight (p = 0.002), BMI (p < 0.001), systolic blood pressure (p < 0.001), diastolic blood pressure (p < 0.001), fasting plasma glucose (p < 0.001), triglyceride (p = 0.004) and LDL cholesterol (p = 0.051). Moreover, we found that genotype distributions were significantly different between groups (p < 0.05) and that the rs895819-C allele is more frequent in controls (p = 0.030, OR = 0.72, 95 % CI 0.53-0.97). CONCLUSION: Our study shows that rs895819 in hsa-mir-27a is associated with T2DM susceptibility and that the C allele conveyed a protective role against T2DM. Larger multicentric and specific functional studies will be necessary to obtain a deeper comprehension of the role of rs895819 and hsa-mir-27a and how they are involved in the development of diabetes.
Entities:
Keywords:
MicroRNA; Single nucleotide polymorphism; Type 2 diabetes mellitus; hsa-mir-27a
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