| Literature DB >> 27295211 |
Geetanjali Kharmate, Elham Hosseini-Beheshti, Josselin Caradec, Mei Yieng Chin, Emma S Tomlinson Guns.
Abstract
[This corrects the article DOI: 10.1371/journal.pone.0154967.].Entities:
Year: 2016 PMID: 27295211 PMCID: PMC4905623 DOI: 10.1371/journal.pone.0157392
Source DB: PubMed Journal: PLoS One ISSN: 1932-6203 Impact factor: 3.240
Fig 4Exosome isolation from plasma is validated by the presence of exosome markers.
a) CD9 was present in exosomes derived from LNCaP xenograft mice bearing small, medium and large LNCaP tumours whereas the control mouse serum lacked CD9. GRP94, a known endoplasmic reticulum protein which is used as a negative control was absent in the exosomes suggesting enrichment b) LAMP2 was present in exosomes derived from PCa patient plasma whereas absence of LAMP2 in whole plasma indicated successful enrichment. c) Alix was present in exosomes derived from patient plasma at different exosomal protein concentrations.