Literature DB >> 27295211

Correction: Epidermal Growth Factor Receptor in Prostate Cancer Derived Exosomes.

Geetanjali Kharmate, Elham Hosseini-Beheshti, Josselin Caradec, Mei Yieng Chin, Emma S Tomlinson Guns.   

Abstract

[This corrects the article DOI: 10.1371/journal.pone.0154967.].

Entities:  

Year:  2016        PMID: 27295211      PMCID: PMC4905623          DOI: 10.1371/journal.pone.0157392

Source DB:  PubMed          Journal:  PLoS One        ISSN: 1932-6203            Impact factor:   3.240


Fig 4A shows expression of CD-9 in small, medium, and large tumor bearing mice. There are erroneously 5 lanes in the figure; the fifth lane was a duplication of large tumor sample from another mouse. Please view the correct Fig 4, without the extraneous lane in panel A, here.
Fig 4

Exosome isolation from plasma is validated by the presence of exosome markers.

a) CD9 was present in exosomes derived from LNCaP xenograft mice bearing small, medium and large LNCaP tumours whereas the control mouse serum lacked CD9. GRP94, a known endoplasmic reticulum protein which is used as a negative control was absent in the exosomes suggesting enrichment b) LAMP2 was present in exosomes derived from PCa patient plasma whereas absence of LAMP2 in whole plasma indicated successful enrichment. c) Alix was present in exosomes derived from patient plasma at different exosomal protein concentrations.

Exosome isolation from plasma is validated by the presence of exosome markers.

a) CD9 was present in exosomes derived from LNCaP xenograft mice bearing small, medium and large LNCaP tumours whereas the control mouse serum lacked CD9. GRP94, a known endoplasmic reticulum protein which is used as a negative control was absent in the exosomes suggesting enrichment b) LAMP2 was present in exosomes derived from PCa patient plasma whereas absence of LAMP2 in whole plasma indicated successful enrichment. c) Alix was present in exosomes derived from patient plasma at different exosomal protein concentrations.

Uncropped blot for Fig 4A.

(TIF) Click here for additional data file.
  1 in total

1.  Epidermal Growth Factor Receptor in Prostate Cancer Derived Exosomes.

Authors:  Geetanjali Kharmate; Elham Hosseini-Beheshti; Josselin Caradec; Mei Yieng Chin; Emma S Tomlinson Guns
Journal:  PLoS One       Date:  2016-05-06       Impact factor: 3.240

  1 in total
  1 in total

Review 1.  Exosomes are the Driving Force in Preparing the Soil for the Metastatic Seeds: Lessons from the Prostate Cancer.

Authors:  Saber H Saber; Hamdy E A Ali; Rofaida Gaballa; Mohamed Gaballah; Hamed I Ali; Mourad Zerfaoui; Zakaria Y Abd Elmageed
Journal:  Cells       Date:  2020-02-28       Impact factor: 6.600

  1 in total

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