Fei Tian1, Yanting Shen2, Zhenzhu Chen2, Rui Li2, Jiafeng Lu3, Qinyu Ge4. 1. Research Center for Learning Science, Southeast University, Nanjing 210096, China; State Key Lab of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China. 2. Research Center for Learning Science, Southeast University, Nanjing 210096, China. 3. State Key Lab of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China. 4. State Key Lab of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China. Electronic address: geqinyu@seu.edu.cn.
Abstract
BACKGROUND: Lung cancer is the leading cause of cancer deaths in China. Non-small cell lung cancer (NSCLC) is the major type of lung cancer. OBJECTIVES: The aim of our study was to characterize the expression profiles of miRNAs in serum and tissue of NSCLC at the same time, and to find more accurate relationship of miRNAs between serum and tissue. Furthermore, we intended to find more biomarkers of miRNAs in NSCLC samples. METHODS: In this study, the miRNAs were sequenced in 18 paired serum and 18 paired tissue samples. The expression levels of miRNAs and targets were quantified by qRT-PCR. The function analysis was performed by using bioinformatics methods. RESULTS: In these paired samples miR-181b-5p was up-regulated in squamous cell carcinoma (SCC), miR-486-5p was down-regulated in adenocarcinoma (AC), and miR-21-5p was up-regulated in both SCC and AC. However, miR-181b-5p and miR-486-5p were rarely reported in lung cancer related studies. The expression levels of these two miRNAs and their targets, RASSF1 and PIK3R1 were quantified in additional samples by qRT-PCR. The results showed that the targets were negatively regulated by the two miRNAs. In addition, we noted that RASSF1 and PIK3R1 were directly involved in non-small cell lung cancer pathway. CONCLUSIONS: Our study suggested that miR-181b-5p and miR-486-5p could be new potential biomarkers for early diagnosis of NSCLC.
BACKGROUND:Lung cancer is the leading cause of cancer deaths in China. Non-small cell lung cancer (NSCLC) is the major type of lung cancer. OBJECTIVES: The aim of our study was to characterize the expression profiles of miRNAs in serum and tissue of NSCLC at the same time, and to find more accurate relationship of miRNAs between serum and tissue. Furthermore, we intended to find more biomarkers of miRNAs in NSCLC samples. METHODS: In this study, the miRNAs were sequenced in 18 paired serum and 18 paired tissue samples. The expression levels of miRNAs and targets were quantified by qRT-PCR. The function analysis was performed by using bioinformatics methods. RESULTS: In these paired samples miR-181b-5p was up-regulated in squamous cell carcinoma (SCC), miR-486-5p was down-regulated in adenocarcinoma (AC), and miR-21-5p was up-regulated in both SCC and AC. However, miR-181b-5p and miR-486-5p were rarely reported in lung cancer related studies. The expression levels of these two miRNAs and their targets, RASSF1 and PIK3R1 were quantified in additional samples by qRT-PCR. The results showed that the targets were negatively regulated by the two miRNAs. In addition, we noted that RASSF1 and PIK3R1 were directly involved in non-small cell lung cancer pathway. CONCLUSIONS: Our study suggested that miR-181b-5p and miR-486-5p could be new potential biomarkers for early diagnosis of NSCLC.
Authors: Wei Hu; Fengqi Yan; Yi Ru; Mingyuan Xia; Guang Yan; Mei Zhang; He Wang; Guojun Wu; Libo Yao; Lan Shen; Xia Li; Qinhao Wang Journal: Am J Cancer Res Date: 2020-02-01 Impact factor: 6.166