Separation of Neutral Glycoasparagines According to Their Content of cis Diol Groups.

A procedure for obtaining pure glycoasparagine specimens was developed. This method constitutes ion-exchange chromatography in a DEAE-Sephadex A-25 (borate form) column, using a linear gradient of borate buffer as the eluent. With no conversion, almost all the individual glycoasparagines, including some new ones, derived from ovalbumin could be isolated in pure form. By the borate-ion exchange chromatographic technique presented here, different types of neutral glycoasparagnes having a differing ability to form a complex with borate ions.