Rat cortical neurons in cell culture: culture methods, cell morphology, electrophysiology, and synapse formation.

Rat cortical neurons from 15 day embryos are grown in dissociated cell culture and maintained in vitro for 8--12 weeks. The neurons develop into forms which resemble mature cortical neurons in situ, stain with silver and exhibit passive and active electrophysiological properties similar to those of cortical neurons. Extensive chemical excitatory and inhibitory synapses develop de novo. These cultures can provide a model for future studies of mammalian CNS neuronal physiology, transmitter pharmacology, pathophysiology and mechanisms of drug action.