Vidyullatha Peddireddy1,2, Siva Prasad Badabagni3, Shehnaz Sulthana4, Venkata Karunakar Kolla3, Sandhya Devi Gundimeda5, Hemaprasad Mundluru3. 1. Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, 500016, Telangana, India. vidyullatha.p@gmail.com. 2. Department of Biotechnology and Bioinformatics, University of Hyderabad, Gachibowli, Hyderabad, 500046, Telangana, India. vidyullatha.p@gmail.com. 3. Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, 500016, Telangana, India. 4. Bhagwan Mahavir Medical and Research Centre, Hyderabad, 500004, Telangana, India. 5. Indo-American Cancer Hospital, Banjara Hills, Hyderabad, 500034, Telangana, India.
Abstract
BACKGROUND: Cytokine-mediated inflammation is important in the pathogenesis of non-small cell lung cancer (NSCLC). Genetic polymorphisms in cytokine genes and their association with lung cancer in the Indian population have not been reported. METHODS: For the first time, we analyzed genetic polymorphisms of TNFα -308, IFNγ +874, and IL10 -1082 genes in 246 NSCLC patients and 250 healthy controls in the South Indian population from Telangana using ARMS PCR. RESULTS: IFNγ+874 A/T and IL10-1082 G/G gene polymorphisms were found to be significantly associated with NSCLC with 1.56- and 1.68-fold disease risk, respectively. There was no association between the risk of NSCLC and TNFα-308 polymorphism. Gene polymorphisms stratified according to smoking revealed that IFNγ+874 A/T polymorphisms in smokers increased the disease risk by 2.91 fold. IL10-1082 G/G polymorphisms showed 2-fold increased risk among patients who were smokers when compared to the controls. However, there was no association between TNFα-308, IFNγ+874, and IL10-1082 gene polymorphism and the stage of the NSCLC patients. The overall risk associated with the combination of these polymorphisms indicated that the TNFα-308 G/A + IFNγ+874 A/T + IL10-1082 G/G genotype increased the risk by 1.5 fold. CONCLUSIONS: The results of our study indicate an association between cytokine gene polymorphisms and the risk of NSCLC in an Indian population.
BACKGROUND: Cytokine-mediated inflammation is important in the pathogenesis of non-small cell lung cancer (NSCLC). Genetic polymorphisms in cytokine genes and their association with lung cancer in the Indian population have not been reported. METHODS: For the first time, we analyzed genetic polymorphisms of TNFα -308, IFNγ +874, and IL10 -1082 genes in 246 NSCLCpatients and 250 healthy controls in the South Indian population from Telangana using ARMS PCR. RESULTS: IFNγ+874 A/T and IL10-1082 G/G gene polymorphisms were found to be significantly associated with NSCLC with 1.56- and 1.68-fold disease risk, respectively. There was no association between the risk of NSCLC and TNFα-308 polymorphism. Gene polymorphisms stratified according to smoking revealed that IFNγ+874 A/T polymorphisms in smokers increased the disease risk by 2.91 fold. IL10-1082 G/G polymorphisms showed 2-fold increased risk among patients who were smokers when compared to the controls. However, there was no association between TNFα-308, IFNγ+874, and IL10-1082 gene polymorphism and the stage of the NSCLCpatients. The overall risk associated with the combination of these polymorphisms indicated that the TNFα-308 G/A + IFNγ+874 A/T + IL10-1082 G/G genotype increased the risk by 1.5 fold. CONCLUSIONS: The results of our study indicate an association between cytokine gene polymorphisms and the risk of NSCLC in an Indian population.
Authors: H Takizawa; M Tanaka; K Takami; T Ohtoshi; K Ito; M Satoh; Y Okada; F Yamasawa; A Umeda Journal: Am J Physiol Lung Cell Mol Physiol Date: 2000-05 Impact factor: 5.464
Authors: E Tarkowski; L Rosengren; C Blomstrand; C Wikkelsö; C Jensen; S Ekholm; A Tarkowski Journal: Clin Exp Immunol Date: 1997-12 Impact factor: 4.330