Bjorn de Wagenaar1, Daan J Geijs1, Hans de Boer1, Johan G Bomer1, Wouter Olthuis1, Albert van den Berg1, Loes I Segerink2. 1. BIOS - Lab on a Chip Group, MESA+ Institute for Nanotechnology and MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, the Netherlands. 2. BIOS - Lab on a Chip Group, MESA+ Institute for Nanotechnology and MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, the Netherlands; Department of Obstetrics and Gynaecology, Radboud University Nijmegen Medical Centre, Nijmegen, the Netherlands. Electronic address: l.i.segerink@utwente.nl.
Abstract
OBJECTIVE: To study single sperm boar motility using electrical impedance measurements in a microfluidic system. DESIGN: Comparison of the optical data and electrical impedance data. SETTING: Research laboratory at a university. ANIMAL(S): Boar semen sample were used. INTERVENTION(S): A microfluidic system is developed that is able to spatially confine single boar sperm cells and allows noninvasive analysis of their motility on the single cell level. Using this system, the single sperm motility was affected by changing the temperature or adding chemical stimuli (caffeine). The retrieved electrical impedance and video data were processed using Matlab. MAIN OUTCOME MEASURE(S): The sperm beat frequency and amplitude determined from the electrical impedance and video data. RESULT(S): The electrically measured sperm beat frequency was verified by optical analysis and in correspondence. Furthermore the microfluidic platform allowed single sperm analysis by altering the sperm by temperature and chemical stimuli. CONCLUSION(S): This platform could be exploited as a potential tool to study sperm cells on the single cell level and to perform advanced sperm selection for intracytoplasmic sperm injection (ICSI) applications.
OBJECTIVE: To study single sperm boar motility using electrical impedance measurements in a microfluidic system. DESIGN: Comparison of the optical data and electrical impedance data. SETTING: Research laboratory at a university. ANIMAL(S): Boar semen sample were used. INTERVENTION(S): A microfluidic system is developed that is able to spatially confine single boar sperm cells and allows noninvasive analysis of their motility on the single cell level. Using this system, the single sperm motility was affected by changing the temperature or adding chemical stimuli (caffeine). The retrieved electrical impedance and video data were processed using Matlab. MAIN OUTCOME MEASURE(S): The sperm beat frequency and amplitude determined from the electrical impedance and video data. RESULT(S): The electrically measured sperm beat frequency was verified by optical analysis and in correspondence. Furthermore the microfluidic platform allowed single sperm analysis by altering the sperm by temperature and chemical stimuli. CONCLUSION(S): This platform could be exploited as a potential tool to study sperm cells on the single cell level and to perform advanced sperm selection for intracytoplasmic sperm injection (ICSI) applications.
Authors: Reza Nosrati; Percival J Graham; Biao Zhang; Jason Riordon; Alexander Lagunov; Thomas G Hannam; Carlos Escobedo; Keith Jarvi; David Sinton Journal: Nat Rev Urol Date: 2017-10-31 Impact factor: 14.432