Tetsuo Kobayashi1, Kohei Ishida2, Hiromasa Yoshie2. 1. Division of Periodontology, Department of Oral Biological Science, Niigata University Graduate School of Medical and Dental Sciences, 2-5274 Gakkocho-dori, Chuo-ku, Niigata 951-8514, Japan; General Dentistry and Clinical Education Unit, Niigata University Medical and Dental Hospital, 2-5274 Gakkocho-dori, Chuo-ku, Niigata 951-8514, Japan. Electronic address: kotetsuo@dent.niigata-u.ac.jp. 2. Division of Periodontology, Department of Oral Biological Science, Niigata University Graduate School of Medical and Dental Sciences, 2-5274 Gakkocho-dori, Chuo-ku, Niigata 951-8514, Japan.
Abstract
OBJECTIVE: DNA methylation of the cytokine genes may play a role in the pathogenesis of periodontitis. The aim of this study is to evaluate whether the alteration of interleukin-6 (IL-6) gene promoter methylation in the gingival tissue (GT) and peripheral blood (PB) is unique to chronic periodontitis (CP). DESIGN: DNA isolated from the GT and PB of 25 patients with (CP) and 20 healthy controls (H) was modified with sodium bisulfite and analyzed for IL-6 promoter methylation with direct sequencing. The levels of IL-6 mRNA and serum IL-6 protein were evaluated by a quantitative reverse transcription polymerase chain reaction and an enzyme-linked immunosorbent assay. RESULTS: The CP group showed that the overall methylation rates of IL-6 promoter that contained 19 cytosine-guanine dinucleotide (CpG) motifs were significantly decreased in GT in comparison to PB (p<0.001), which was significantly negatively correlated with the probing depth (p=0.003). The GT and PB of the H group displayed similar overall methylation rates. No significant difference was observed in the methylation rates at each CpG in GT in comparison to the PB in both groups. The levels of IL-6 mRNA in the GT and PB and serum IL-6 of the two groups were comparable. The ratio of IL-6 mRNA in the GT relative to the PB was significantly higher in the CP group than in the H group (p=0.03). CONCLUSION: The increased expression of IL-6 gene transcription may be related to IL-6 promoter hypomethylation in the GT from CP patients.
OBJECTIVE: DNA methylation of the cytokine genes may play a role in the pathogenesis of periodontitis. The aim of this study is to evaluate whether the alteration of interleukin-6 (IL-6) gene promoter methylation in the gingival tissue (GT) and peripheral blood (PB) is unique to chronic periodontitis (CP). DESIGN: DNA isolated from the GT and PB of 25 patients with (CP) and 20 healthy controls (H) was modified with sodium bisulfite and analyzed for IL-6 promoter methylation with direct sequencing. The levels of IL-6 mRNA and serum IL-6 protein were evaluated by a quantitative reverse transcription polymerase chain reaction and an enzyme-linked immunosorbent assay. RESULTS: The CP group showed that the overall methylation rates of IL-6 promoter that contained 19 cytosine-guanine dinucleotide (CpG) motifs were significantly decreased in GT in comparison to PB (p<0.001), which was significantly negatively correlated with the probing depth (p=0.003). The GT and PB of the H group displayed similar overall methylation rates. No significant difference was observed in the methylation rates at each CpG in GT in comparison to the PB in both groups. The levels of IL-6 mRNA in the GT and PB and serum IL-6 of the two groups were comparable. The ratio of IL-6 mRNA in the GT relative to the PB was significantly higher in the CP group than in the H group (p=0.03). CONCLUSION: The increased expression of IL-6 gene transcription may be related to IL-6 promoter hypomethylation in the GT from CP patients.
Authors: Dessie Salilew-Wondim; Mohammed Saeed-Zidane; Michael Hoelker; Samuel Gebremedhn; Mikhaël Poirier; Hari Om Pandey; Ernst Tholen; Christiane Neuhoff; Eva Held; Urban Besenfelder; Vita Havlicek; Franca Rings; Eric Fournier; Dominic Gagné; Marc-André Sirard; Claude Robert; Ahmed Gad; Karl Schellander; Dawit Tesfaye Journal: BMC Genomics Date: 2018-06-01 Impact factor: 3.969
Authors: Yuko Kurushima; Pei-Chien Tsai; Juan Castillo-Fernandez; Alexessander Couto Alves; Julia Sarah El-Sayed Moustafa; Caroline Le Roy; Tim D Spector; Mark Ide; Francis J Hughes; Kerrin S Small; Claire J Steves; Jordana T Bell Journal: Clin Epigenetics Date: 2019-02-13 Impact factor: 6.551
Authors: P-J Almiñana-Pastor; M Boronat-Catalá; P Micó-Martinez; C Bellot-Arcís; A Lopez-Roldan; F-M Alpiste-Illueca Journal: Med Oral Patol Oral Cir Bucal Date: 2019-09-01
Authors: R M Díaz Sánchez; G Castillo-Dalí; A Fernández-Olavarría; R Mosquera-Pérez; J M Delgado-Muñoz; J L Gutiérrez-Pérez; D Torres-Lagares Journal: Mediators Inflamm Date: 2017-12-10 Impact factor: 4.711