Ryohei Ogawa1, Akihiro Morii2, Akihiko Watanabe2. 1. Department of Radiological Sciences, Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama, 2630 Sugitani, Toyama, 930-0194, Japan. ogawa@med.u-toyama.ac.jp. 2. Department of Urology, Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama, Toyama, Japan.
Abstract
PURPOSE: The purpose of this study is to investigate the involvement of microRNAs (miRNAs) in sonication-induced apoptosis. METHODS: U937 cells derived from human leukemia were sonicated with 1-MHz ultrasound at 0.4 W/cm(2) and 10 % duty factor for 60 s, a condition inducing apoptosis. The total RNA was extracted from cells at various timings after sonication and subjected to microarray and real-time PCR for miRNA expression analyses. RESULTS: Expression of several miRNAs was significantly affected by sonication. For miR-424* and miR-720, whose expressions were eminently decreased by sonication, cell lines overexpressing these miRNAs were established. Conversely, for miR-663B and miR-663, whose expressions were eminently increased by sonication, cell lines inhibiting these miRNA functions were established. When these cell lines were sonicated, a cell line inhibiting miR-663B function significantly increased sonication-induced apoptosis, suggesting this may be involved in cellular responses to sonication. Two genes that could induce apoptosis, KSR2 and CREBZF, were identified as potential target genes of miR-663B since potential target sequences on their 3' UTR mediated to decrease expression of a reporter gene. CONCLUSION: These results suggest that miRNAs may be involved in cellular responses to ultrasound through their expression changes caused by sonication.
PURPOSE: The purpose of this study is to investigate the involvement of microRNAs (miRNAs) in sonication-induced apoptosis. METHODS: U937 cells derived from humanleukemia were sonicated with 1-MHz ultrasound at 0.4 W/cm(2) and 10 % duty factor for 60 s, a condition inducing apoptosis. The total RNA was extracted from cells at various timings after sonication and subjected to microarray and real-time PCR for miRNA expression analyses. RESULTS: Expression of several miRNAs was significantly affected by sonication. For miR-424* and miR-720, whose expressions were eminently decreased by sonication, cell lines overexpressing these miRNAs were established. Conversely, for miR-663B and miR-663, whose expressions were eminently increased by sonication, cell lines inhibiting these miRNA functions were established. When these cell lines were sonicated, a cell line inhibiting miR-663B function significantly increased sonication-induced apoptosis, suggesting this may be involved in cellular responses to sonication. Two genes that could induce apoptosis, KSR2 and CREBZF, were identified as potential target genes of miR-663B since potential target sequences on their 3' UTR mediated to decrease expression of a reporter gene. CONCLUSION: These results suggest that miRNAs may be involved in cellular responses to ultrasound through their expression changes caused by sonication.
Authors: H Ashush; L A Rozenszajn; M Blass; M Barda-Saad; D Azimov; J Radnay; D Zipori; U Rosenschein Journal: Cancer Res Date: 2000-02-15 Impact factor: 12.701
Authors: Amir Abdollahi; Sophie Domhan; Juergen W Jenne; Mazin Hallaj; Giorgio Dell'Aqua; Martina Mueckenthaler; Alexandra Richter; Heather Martin; Juergen Debus; Wilhelm Ansorge; Kullervo Hynynen; Peter E Huber Journal: FASEB J Date: 2004-07-01 Impact factor: 5.191