Marco Gandini1, Simona Cerri2, Paola Pregel3, Gessica Giusto4, Cristina Vercelli5, Selina Iussich6, Massimiliano Tursi6, Anna Maria Farca7. 1. Assistant Professor, Department of Veterinary Sciences, University of Turin, Italy. Design of the study; technical procedures; acquisition, interpretation and analysis of data; manuscript preparation and writing., Department of Veterinary Sciences, University of Turin, Italy. 2. DVM, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liege, Belgium. Technical procedures; acquisition, interpretation and analysis of data; manuscript preparation and writing., Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liege, Belgium. 3. MS, PhD, Department of Veterinary Sciences, University of Turin, Italy. Analysis and interpretation of data, critical revision., Department of Veterinary Sciences, University of Turin, Italy. 4. DVM, PhD, Department of Veterinary Sciences, University of Turin, Italy. Manuscript writing, critical revision., Department of Veterinary Sciences, University of Turin, Italy. 5. MS, PhD, Department of Veterinary Sciences, University of Turin, Italy. Interpretation and analysis of data, manuscript preparation and writing., Department of Veterinary Sciences, University of Turin, Italy. 6. Assistant Professor, Department of Veterinary Sciences, University of Turin, Italy. Histopathological examination, critical revision., Department of Veterinary Sciences, University of Turin, Italy. 7. Full Professor, Department of Veterinary Sciences, University of Turin, Italy. Design of the study, interpretation and analysis of data, manuscript preparation and writing., Department of Veterinary Sciences, University of Turin, Italy.
Abstract
PURPOSE: To evaluate the effects of an intraperitoneal solution of methylene blue (MB), lidocaine and pentoxyphylline (PTX) on intestinal ischemic and reperfusion injury. METHODS: Superior mesenteric artery was isolated and clamped in 36 adult male Sprague Dawley rats. After 60 minutes, clamp was removed and a group received intraperitoneally UNITO solution (PTX 25mg/kg + lidocaine 5mg/kg + MB 2mg/kg), while the other group was treated with warm 0.9% NaCl solution. Rats were euthanized 45 min after drug administration. Lung and bowel were collected for histological evaluation (using Park's score) and determination of myeloperoxidase (MPO) and malondialdehyde (MDA) levels. RESULTS: Control samples showed lymphoplasmocytic infiltrate and crypt necrosis of villi. MPO and MDA measurements shown no differences between treated and control groups. CONCLUSION: The combination of lidocaine, methylene blue and pentoxyphylline administered intraperitoneally at the studied dose, did not decreased histological lesion scores and biochemical markers levels in intestinal ischemia/reperfusion injury.
PURPOSE: To evaluate the effects of an intraperitoneal solution of methylene blue (MB), lidocaine and pentoxyphylline (PTX) on intestinal ischemic and reperfusion injury. METHODS: Superior mesenteric artery was isolated and clamped in 36 adult male Sprague Dawley rats. After 60 minutes, clamp was removed and a group received intraperitoneally UNITO solution (PTX 25mg/kg + lidocaine 5mg/kg + MB 2mg/kg), while the other group was treated with warm 0.9% NaCl solution. Rats were euthanized 45 min after drug administration. Lung and bowel were collected for histological evaluation (using Park's score) and determination of myeloperoxidase (MPO) and malondialdehyde (MDA) levels. RESULTS: Control samples showed lymphoplasmocytic infiltrate and crypt necrosis of villi. MPO and MDA measurements shown no differences between treated and control groups. CONCLUSION: The combination of lidocaine, methylene blue and pentoxyphylline administered intraperitoneally at the studied dose, did not decreased histological lesion scores and biochemical markers levels in intestinal ischemia/reperfusion injury.
Authors: Juan Morgaz; Sergio Ventura; Pilar Muñoz-Rascón; Rocio Navarrete; José Pérez; María Del Mar Granados; José Andrés Fernández-Sarmiento; Juan Manuel Domínguez; Verónica Molina; Rafael J Gómez-Villamandos; Rafael Zafra Journal: BMC Vet Res Date: 2020-02-12 Impact factor: 2.741