Lobna S Sherif1, Randaa K Abdel Raouf2, Rokaya M El Sayede3, Amany S El Wakkadd4, Ashraf R Shoaib5, Hanan M Ali6, Amira S El Refay1. 1. National Research Centre, Department of Child Health, Giza, Egypt. 2. Institute of Postgraduate Childhood Studies - Department of Medical Studies, Cairo, Egypt. 3. Kasralainy Cairo University - Pediatric, Cairo, Egypt. 4. National Research Centre, Medical Physiology, Cairo, Egypt. 5. National Research Centre, Department of Virology, Giza, Egypt. 6. National Research Centre, Department of Cell Biology, Giza, Egypt.
Abstract
BACKGROUND: Secretory immunoglobulin A (SIgA) plays an important protective role in the recognition and clearance of enteric pathogens. AIM: This study was designed to assess if mucosal integrity "measured by secretory IgA (SIgA)" is a protective factor from more epithelial alteration "measured by glutathione transferase" in infants with Rota gastroenteritis and its relation to infants' feeding pattern. PATIENTS AND METHODS: This study was conducted on 79 infants aged 6 months and less from those diagnosed as having gastroenteritis and admitted to Gastroenteritis Department in Abo El Rish Pediatric Hospital, Cairo University. Plasma glutathione s-transferases and Stool SIgA were measured using ELISA technique. Rota virus detection was done by Reverse transcriptase PCR. RESULTS: SIgA was found to be significantly positive in exclusive breast fed infants, Glutathione transferase was significantly more frequently positive in Rota positive cases than Rota negative cases by Reverse transcriptase PCR. A significant negative correlation between Glutathione transferase and Secretory IgA was found, (p < 0.05). CONCLUSION: Breast feeding should be encouraged and highly recommended in the first two years of life as it provides Secretory IgA to breast fed infants who in turn protect them against epithelial damage caused by Rota viral gastroenteritis.
BACKGROUND: Secretory immunoglobulin A (SIgA) plays an important protective role in the recognition and clearance of enteric pathogens. AIM: This study was designed to assess if mucosal integrity "measured by secretory IgA (SIgA)" is a protective factor from more epithelial alteration "measured by glutathione transferase" in infants with Rota gastroenteritis and its relation to infants' feeding pattern. PATIENTS AND METHODS: This study was conducted on 79 infants aged 6 months and less from those diagnosed as having gastroenteritis and admitted to Gastroenteritis Department in Abo El Rish Pediatric Hospital, Cairo University. Plasma glutathione s-transferases and Stool SIgA were measured using ELISA technique. Rota virus detection was done by Reverse transcriptase PCR. RESULTS: SIgA was found to be significantly positive in exclusive breast fed infants, Glutathione transferase was significantly more frequently positive in Rota positive cases than Rota negative cases by Reverse transcriptase PCR. A significant negative correlation between Glutathione transferase and Secretory IgA was found, (p < 0.05). CONCLUSION: Breast feeding should be encouraged and highly recommended in the first two years of life as it provides Secretory IgA to breast fed infants who in turn protect them against epithelial damage caused by Rota viral gastroenteritis.
Authors: A J Macpherson; A Lamarre; K McCoy; G R Harriman; B Odermatt; G Dougan; H Hengartner; R M Zinkernagel Journal: Nat Immunol Date: 2001-07 Impact factor: 25.606
Authors: Morgan P McMonagle; Michelle Halpenny; Annette McCarthy; Alan Mortell; Fiona Manning; Cormac Kilty; David Mannion; Alfred E Wood; Martin T Corbally Journal: J Pediatr Surg Date: 2006-09 Impact factor: 2.545
Authors: Mathew D Esona; Rashi Gautam; Ka Ian Tam; Alice Williams; Slavica Mijatovic-Rustempasic; Michael D Bowen Journal: J Virol Methods Date: 2015-07-29 Impact factor: 2.014
Authors: Umesh D Parashar; Erik G Hummelman; Joseph S Bresee; Mark A Miller; Roger I Glass Journal: Emerg Infect Dis Date: 2003-05 Impact factor: 6.883