Xingpei Hao1, Hang Xiao2, Jihyueng Ju2, Stephen M Hewitt3, Herbert C Morse4. 1. Susan Lehman Cullman laboratory for Cancer Research, Department of Chemical Biology, Earnest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ, U.S.A. xhao@mail.nih.gov. 2. Susan Lehman Cullman laboratory for Cancer Research, Department of Chemical Biology, Earnest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ, U.S.A. 3. Laboratory of Pathology, National Cancer Institute, Bethesda, MD, U.S.A. 4. Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, Rockville, MD, U.S.A.
Abstract
BACKGROUND/AIM: The family of retinoid X receptors (RXRs) including RXRα, β and γ, is involved in regulating cell proliferation, differentiation, apoptosis and development. MATERIALS AND METHODS: In order to characterize the role of RXRs during colorectal carcinogenesis, the expression of RXRs in human and azoxymethane (AOM)-induced rat colorectal tumors was profiled by immunohistochemistry. RESULTS: Both human and rat normal colorectal epithelia and hyperplasia exhibited strong nuclear, but weak cytoplasmic staining for all three proteins. Expression of RXRα, β and γ was significantly reduced in rat carcinomas compared to high-grade dysplasia whether in aberrant crypt foci or in adenomas. All three proteins displayed dramatically reduced nuclear expression in both human adenomas and carcinomas. Reduced expression of RXRα and RXRγ seems more significant than RXRβ in both human and rat carcinomas. CONCLUSION: Reduced expression of RXRs is associated with colorectal carcinogenesis in both humans and AOM-treated rats. Copyright
BACKGROUND/AIM: The family of retinoid X receptors (RXRs) including RXRα, β and γ, is involved in regulating cell proliferation, differentiation, apoptosis and development. MATERIALS AND METHODS: In order to characterize the role of RXRs during colorectal carcinogenesis, the expression of RXRs in human and azoxymethane (AOM)-induced ratcolorectal tumors was profiled by immunohistochemistry. RESULTS: Both human and rat normal colorectal epithelia and hyperplasia exhibited strong nuclear, but weak cytoplasmic staining for all three proteins. Expression of RXRα, β and γ was significantly reduced in ratcarcinomas compared to high-grade dysplasia whether in aberrant crypt foci or in adenomas. All three proteins displayed dramatically reduced nuclear expression in both humanadenomas and carcinomas. Reduced expression of RXRα and RXRγ seems more significant than RXRβ in both human and ratcarcinomas. CONCLUSION: Reduced expression of RXRs is associated with colorectal carcinogenesis in both humans and AOM-treated rats. Copyright
Authors: Y J Wan; D An; Y Cai; J J Repa; T Hung-Po Chen; M Flores; C Postic; M A Magnuson; J Chen; K R Chien; S French; D J Mangelsdorf; H M Sucov Journal: Mol Cell Biol Date: 2000-06 Impact factor: 4.272
Authors: Y Muto; H Moriwaki; M Ninomiya; S Adachi; A Saito; K T Takasaki; T Tanaka; K Tsurumi; M Okuno; E Tomita; T Nakamura; T Kojima Journal: N Engl J Med Date: 1996-06-13 Impact factor: 91.245
Authors: Rodolfo Ocadiz-Delgado; Eduardo Castañeda-Saucedo; Arup K Indra; Rogelio Hernandez-Pando; Pedro Flores-Guizar; Jose Luis Cruz-Colin; Felix Recillas-Targa; Guillermo Perez-Ishiwara; Luis Covarrubias; Patricio Gariglio Journal: Cancer Lett Date: 2011-11-29 Impact factor: 8.679
Authors: C Giaginis; I Koutsounas; P Alexandrou; A Zizi-Serbetzoglou; E Patsouris; G Kouraklis; S Theocharis Journal: Neoplasma Date: 2015 Impact factor: 2.575
Authors: Hang Xiao; Xingpei Hao; Barbara Simi; Jihyeung Ju; Heyuan Jiang; Bandaru S Reddy; Chung S Yang Journal: Carcinogenesis Date: 2007-09-24 Impact factor: 4.944
Authors: Bryan R Haugen; Lori Lee Larson; Umarani Pugazhenthi; William R Hays; Joshua P Klopper; Cynthia A Kramer; Vibha Sharma Journal: J Clin Endocrinol Metab Date: 2004-01 Impact factor: 5.958