| Literature DB >> 27271911 |
Xuehua Xu1, Michelle Yun2, Xi Wen2, Joseph Brzostowski3, Wei Quan2, Q Jane Wang4, Tian Jin5.
Abstract
Receptor activation upon ligand binding induces activation of multiple signaling pathways. To fully understand how these signaling pathways coordinate, it is essential to determine the dynamic nature of the spatiotemporal activation profile of signaling components at the level of single living cells. Here, we outline a detailed methodology for visualizing and quantitatively measuring the spatiotemporal activation of Ras and PKD1 by applying advanced fluorescence imaging techniques, including multichannel, simultaneous imaging and Förster resonance energy transfer (FRET).Entities:
Keywords: Confocal fluorescence microscopy; Förster resonance energy transfer (FRET); Phosphorylation of PKD1; Protein kinase D 1 (PKD1); Ras activation
Mesh：
Substances：
Year: 2016 PMID： 27271911 PMCID： PMC5500199 DOI： 10.1007/978-1-4939-3480-5_22
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745