Sumin Wang1,2,3, Yewei Huang1,2,3, Huanhuan Xu1,2,3, Qiangqiang Zhu1,2,3, Hao Lu1,2,3, Mengmeng Zhang1,2,3, Shumei Hao4, Chongye Fang1,2,3, Dongying Zhang1,2,3, Xiaoyun Wu5,6,7, Xuanjun Wang8,9,10,11, Jun Sheng12,13,14,15. 1. Key Laboratory of Puer Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming, 650201, China. 2. Yunnan Research Center for Tea Processing, Yunnan Agricultural University, Kunming, 650201, China. 3. Yunnan Station of Tea Resource and Processing, Ministry of Agriculture, Kunming, 650201, China. 4. Department of Life Science, Yunnan University, Kunming, 650091, China. 5. Key Laboratory of Puer Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming, 650201, China. wuxiaoyun79@gmail.com. 6. Yunnan Research Center for Tea Processing, Yunnan Agricultural University, Kunming, 650201, China. wuxiaoyun79@gmail.com. 7. Yunnan Station of Tea Resource and Processing, Ministry of Agriculture, Kunming, 650201, China. wuxiaoyun79@gmail.com. 8. Key Laboratory of Puer Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming, 650201, China. wangxuanjun@gmail.com. 9. Yunnan Research Center for Tea Processing, Yunnan Agricultural University, Kunming, 650201, China. wangxuanjun@gmail.com. 10. Yunnan Station of Tea Resource and Processing, Ministry of Agriculture, Kunming, 650201, China. wangxuanjun@gmail.com. 11. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, 650201, China. wangxuanjun@gmail.com. 12. Key Laboratory of Puer Tea Science, Ministry of Education, Yunnan Agricultural University, Kunming, 650201, China. shengj@ynau.edu.cn. 13. Yunnan Research Center for Tea Processing, Yunnan Agricultural University, Kunming, 650201, China. shengj@ynau.edu.cn. 14. Yunnan Station of Tea Resource and Processing, Ministry of Agriculture, Kunming, 650201, China. shengj@ynau.edu.cn. 15. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, 650201, China. shengj@ynau.edu.cn.
Abstract
BACKGROUND: Tea polyphenols are the prominent component in tea. After the fermentation process, tea polyphenols are oxidized by polyphenol oxidase to form oxidized tea polyphenols (OTPs). OTPs contain a significant amount of hydrophobic phenyl groups that can bind with non-aqueous materials. Here, we determined whether OTPs can bind with lipids and reduce fat uptake and assessed the effect of OTPs on decreasing obesity and alleviating hyperlipidaemia and other metabolic syndromes. METHODS: Rats were divided into three groups: control, high-fat diet (HFD) and OTP groups. The control and HFD groups were fed a chow diet and a high-fat diet, respectively, for 12 weeks; the OTP group was fed a high-fat diet for 6 weeks and then a high-fat diet containing 2 % OTP for 6 weeks. The serum and excrement triglyceride (TAG) and total cholesterol (CHOL) concentrations were determined, and liver tissue and white adipose tissue were collected to detect the expression levels of genes involved in lipid metabolism. RESULTS: Our results revealed that OTPs failed to decrease the serum concentrations of TAG and CHOL. OTPs alleviated the accumulation of lipids in the liver tissue and changed the expression levels of the regulators of lipid metabolism, i.e., peroxisome proliferation-activated receptors (ppars), compared with the rats fed a high-fat diet alone. We also observed a significantly decreased reduction of weight in the visceral white adipose, enhanced regulation of fatty acid β-oxidation by PPARα and enhanced biosynthesis of mitochondria in the visceral white adipose of the OTP rats compared with the HFD rats. Additionally, OTPs promoted the excretion of lipids. CONCLUSION: Our results suggest that OTPs alleviate the accumulation of lipids in liver and visceral white adipose tissue and promote lipid excretion in rats in vivo.
BACKGROUND: Tea polyphenols are the prominent component in tea. After the fermentation process, tea polyphenols are oxidized by polyphenol oxidase to form oxidized tea polyphenols (OTPs). OTPs contain a significant amount of hydrophobic phenyl groups that can bind with non-aqueous materials. Here, we determined whether OTPs can bind with lipids and reduce fat uptake and assessed the effect of OTPs on decreasing obesity and alleviating hyperlipidaemia and other metabolic syndromes. METHODS:Rats were divided into three groups: control, high-fat diet (HFD) and OTP groups. The control and HFD groups were fed a chow diet and a high-fat diet, respectively, for 12 weeks; the OTP group was fed a high-fat diet for 6 weeks and then a high-fat diet containing 2 % OTP for 6 weeks. The serum and excrement triglyceride (TAG) and total cholesterol (CHOL) concentrations were determined, and liver tissue and white adipose tissue were collected to detect the expression levels of genes involved in lipid metabolism. RESULTS: Our results revealed that OTPs failed to decrease the serum concentrations of TAG and CHOL. OTPs alleviated the accumulation of lipids in the liver tissue and changed the expression levels of the regulators of lipid metabolism, i.e., peroxisome proliferation-activated receptors (ppars), compared with the rats fed a high-fat diet alone. We also observed a significantly decreased reduction of weight in the visceral white adipose, enhanced regulation of fatty acid β-oxidation by PPARα and enhanced biosynthesis of mitochondria in the visceral white adipose of the OTPrats compared with the HFD rats. Additionally, OTPs promoted the excretion of lipids. CONCLUSION: Our results suggest that OTPs alleviate the accumulation of lipids in liver and visceral white adipose tissue and promote lipid excretion in rats in vivo.
Authors: Janna A van Diepen; Rinke Stienstra; Irene O C M Vroegrijk; Sjoerd A A van den Berg; Daniela Salvatori; Guido J Hooiveld; Sander Kersten; Cees J Tack; Mihai G Netea; Johannes W A Smit; Leo A B Joosten; Louis M Havekes; Ko Willems van Dijk; Patrick C N Rensen Journal: J Lipid Res Date: 2012-11-17 Impact factor: 5.922
Authors: F Concha; G Prado; J Quezada; A Ramirez; N Bravo; C Flores; J J Herrera; N Lopez; D Uribe; L Duarte-Silva; P Lopez-Legarrea; Diego F Garcia-Diaz Journal: Rev Endocr Metab Disord Date: 2019-06 Impact factor: 6.514