| Literature DB >> 27270396 |
R P B Melo1, J C Almeida1, D C V Lima1, C M Pedrosa1, F J R Magalhães1, A M Alcântara1, L D Barros2, R F C Vieira3, J L Garcia2, R A Mota4.
Abstract
Toxoplasma gondii isolates from Brazil have a different phenotypic and genotypic pattern, with predominance of virulent isolates and recombinant genotypes, compared to the North Hemisphere. Considering that a new T. gondii genotype, non-pathogenic to mice, was previously identified from free-range chickens from the Fernando de Noronha Island, Brazil, this study aimed to identify genotypes of this parasite in tissue samples of feral cats (Felis catus) from this Brazilian Island. Anti-T. gondii IgG antibodies were detected in 18/31 (58%) feral cats. Two non-virulent T. gondii isolates were obtained by mouse bioassay. Genotyping was performed by PCR-RFLP using 10 genetic markers (SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, PK1, L358 and Apico) and an atypical strain of T. gondii (ToxoDB #146) was identified. This is the first report of this genotype in feral cats.Entities:
Keywords: Brazil; Felis catus; Genotyping; Mouse bioassay; Toxoplasmosis
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Year: 2016 PMID： 27270396 DOI： 10.1016/j.vetpar.2016.05.023
Source DB: PubMed Journal: Vet Parasitol ISSN： 0304-4017 Impact factor: 2.738