Qingzhong Wang1, Yogesh Dwivedi1. 1. a Department of Psychiatry and Behavioral Neurobiology , University of Alabama at Birmingham , Birmingham , AL , USA.
Abstract
OBJECTIVES: Recent evidences suggest that mitochondrial dysfunction maybe involved in the pathophysiology of major depressive disorder (MDD); however, the role of mitochondrial genes in this disorder has not been studied systematically. In the present study, we profiled expression of mitochondrial genes in dorsolateral prefrontal cortex (dlPFC) of MDD and non-psychiatric control subjects. METHODS: Human mitochondrial RT2 profile PCR array plates were used to examine differentially expressed genes in dlPFC of 11 MDD and 11 control subjects. Differentially expressed genes were validated independently by qRT-PCR. Biological relevance of differentially expressed genes was analysed by gene ontology (GO) and ingenuity pathways analysis (IPA). RESULTS: We found that 16 genes were differentially expressed in the MDD group compared with control group. Among them, three genes were downregulated and 13 genes upregulated. None of these genes were affected by confounding variables, such as age, post-mortem interval, brain pH, and antidepressant toxicology. Seven differentially expressed genes were successfully validated in MDD subjects. GO and IPA analyses identified several new regulatory networks associated with mitochondrial dysfunctions in MDD. CONCLUSIONS: Our findings suggest abnormal mitochondrial systems in the brain of MDD subjects which could be involved in the etiopathogenesis of this disorder.
OBJECTIVES: Recent evidences suggest that mitochondrial dysfunction maybe involved in the pathophysiology of major depressive disorder (MDD); however, the role of mitochondrial genes in this disorder has not been studied systematically. In the present study, we profiled expression of mitochondrial genes in dorsolateral prefrontal cortex (dlPFC) of MDD and non-psychiatric control subjects. METHODS:Human mitochondrial RT2 profile PCR array plates were used to examine differentially expressed genes in dlPFC of 11 MDD and 11 control subjects. Differentially expressed genes were validated independently by qRT-PCR. Biological relevance of differentially expressed genes was analysed by gene ontology (GO) and ingenuity pathways analysis (IPA). RESULTS: We found that 16 genes were differentially expressed in the MDD group compared with control group. Among them, three genes were downregulated and 13 genes upregulated. None of these genes were affected by confounding variables, such as age, post-mortem interval, brain pH, and antidepressant toxicology. Seven differentially expressed genes were successfully validated in MDD subjects. GO and IPA analyses identified several new regulatory networks associated with mitochondrial dysfunctions in MDD. CONCLUSIONS: Our findings suggest abnormal mitochondrial systems in the brain of MDD subjects which could be involved in the etiopathogenesis of this disorder.
Authors: Galina T Shishkina; Tatyana S Kalinina; Inna V Berezova; Veta V Bulygina; Nikolay N Dygalo Journal: Behav Brain Res Date: 2010-05-08 Impact factor: 3.332
Authors: Yogesh Dwivedi; Hooriyah S Rizavi; Tara Teppen; Hui Zhang; Amal Mondal; Rosalinda C Roberts; Robert R Conley; Ghanshyam N Pandey Journal: Neuropsychopharmacology Date: 2007-12-12 Impact factor: 7.853
Authors: Michael Maes; Piotr Galecki; Yong Seun Chang; Michael Berk Journal: Prog Neuropsychopharmacol Biol Psychiatry Date: 2010-05-12 Impact factor: 5.067
Authors: Gislaine T Rezin; Mariane R Cardoso; Cinara L Gonçalves; Giselli Scaini; Daiane B Fraga; Rafael E Riegel; Clarissa M Comim; João Quevedo; Emilio L Streck Journal: Neurochem Int Date: 2008-09-27 Impact factor: 3.921
Authors: Mandakh Bekhbat; David R Goldsmith; Bobbi J Woolwine; Ebrahim Haroon; Andrew H Miller; Jennifer C Felger Journal: Mol Psychiatry Date: 2021-09-17 Impact factor: 15.992