Dina Ragab1, Dina Soliman2, Dalia Samaha3, Aisha Yassin4. 1. Clinical and Chemical Pathology, Faculty of Medicine, Ain Shams University, Egypt. Electronic address: dinaragab@med.asu.edu.eg. 2. Clinical and Chemical Pathology, Faculty of Medicine, Ain Shams University, Egypt. Electronic address: dinasoliman75@yahoo.com. 3. Clinical and Chemical Pathology, Faculty of Medicine, Ain Shams University, Egypt. Electronic address: daliasamaha@hotmail.com. 4. Clinical and Chemical Pathology, Faculty of Medicine, Ain Shams University, Egypt. Electronic address: shooshoo42@hotmail.com.
Abstract
OBJECTIVES: We aimed to investigate the influence of vitamin D on the production of the pro-inflammatory cytokine, interferon gamma (IFN-γ), and the anti-inflammatory cytokine, interleukin-10 (IL-10), in peripheral blood mononuclear cell (PBMC) cultures. METHODS: Thirty healthy subjects were investigated. Serum levels of calcium, 25(OH) D, and parathyroid hormone (PTH) were assessed. PBMCs were activated in-vitro by phytohemagglutinin (PHA) in the presence and absence of vitamin D3 and then levels of IFN-γ and IL-10 were determined in culture supernatant using enzyme immunoassay. RESULTS: Serum calcium levels were significantly lower in the vitamin D deficient group while serum PTH levels were significantly higher in the vitamin D deficient group. PTH levels were inversely correlated to both calcium and 25(OH) D levels. In culture, vitamin D inhibited IFN-γ production and increased IL-10 production by PBMCs. Serum vitamin D status had no influence on the amount of cytokine produced in culture. CONCLUSIONS: This study demonstrates that vitamin D modulates IFN-γ and IL-10 production and provides a rationale for evaluating vitamin D as an immunomodulatory agent.
OBJECTIVES: We aimed to investigate the influence of vitamin D on the production of the pro-inflammatory cytokine, interferon gamma (IFN-γ), and the anti-inflammatory cytokine, interleukin-10 (IL-10), in peripheral blood mononuclear cell (PBMC) cultures. METHODS: Thirty healthy subjects were investigated. Serum levels of calcium, 25(OH) D, and parathyroid hormone (PTH) were assessed. PBMCs were activated in-vitro by phytohemagglutinin (PHA) in the presence and absence of vitamin D3 and then levels of IFN-γ and IL-10 were determined in culture supernatant using enzyme immunoassay. RESULTS: Serum calcium levels were significantly lower in the vitamin D deficient group while serum PTH levels were significantly higher in the vitamin D deficient group. PTH levels were inversely correlated to both calcium and 25(OH) D levels. In culture, vitamin D inhibited IFN-γ production and increased IL-10 production by PBMCs. Serum vitamin D status had no influence on the amount of cytokine produced in culture. CONCLUSIONS: This study demonstrates that vitamin D modulates IFN-γ and IL-10 production and provides a rationale for evaluating vitamin D as an immunomodulatory agent.
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