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Literature DB >> 27268382

Palladium-Assisted Removal of a Solubilizing Tag from a Cys Side Chain To Facilitate Peptide and Protein Synthesis.

Suman Kumar Maity¹, Guy Mann¹, Muhammad Jbara¹, Shay Laps¹, Guy Kamnesky¹, Ashraf Brik¹.

Abstract

Reversible attachment of solubilizing tags to hydrophobic peptides to facilitate their purification and ligation is an essential yet challenging task in chemical protein synthesis. The efficient palladium-assisted removal of the solubilizing tag linked to the Cys side chain is reported. The strategy was applied for the efficient preparation of histone protein H4 from two fragments via one-pot operation of ligation, removal of the solubilizing tag, and desulfurization.

Entities: Chemical Gene

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Year: 2016 PMID： 27268382 DOI： 10.1021/acs.orglett.6b01442

Source DB: PubMed Journal: Org Lett ISSN： 1523-7052 Impact factor: 6.005

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Cited

17 in total

1. Chemical synthesis of membrane proteins by the removable backbone modification method.

Authors: Shan Tang; Chao Zuo; Dong-Liang Huang; Xiao-Ying Cai; Long-Hua Zhang; Chang-Lin Tian; Ji-Shen Zheng; Lei Liu
Journal: Nat Protoc Date: 2017-11-16 Impact factor: 13.491

2. Palladium-Protein Oxidative Addition Complexes by Amine-Selective Acylation.

Authors: Heemal H Dhanjee; Ivan Buslov; Ian W Windsor; Ronald T Raines; Bradley L Pentelute; Stephen L Buchwald
Journal: J Am Chem Soc Date: 2020-12-15 Impact factor: 15.419

3. Site-Specific Incorporation of Selenocysteine Using an Expanded Genetic Code and Palladium-Mediated Chemical Deprotection.

Authors: Jun Liu; Feng Zheng; Rujin Cheng; Shanshan Li; Sharon Rozovsky; Qian Wang; Lei Wang
Journal: J Am Chem Soc Date: 2018-07-09 Impact factor: 15.419

Palladium-Assisted Removal of a Solubilizing Tag from a Cys Side Chain To Facilitate Peptide and Protein Synthesis.

1. Chemical synthesis of membrane proteins by the removable backbone modification method.

2. Palladium-Protein Oxidative Addition Complexes by Amine-Selective Acylation.

3. Site-Specific Incorporation of Selenocysteine Using an Expanded Genetic Code and Palladium-Mediated Chemical Deprotection.

Review 4. Aligator: A computational tool for optimizing total chemical synthesis of large proteins.

5. Total chemical synthesis of histones and their analogs, assisted by native chemical ligation and palladium complexes.

6. A glutamic acid-based traceless linker to address challenging chemical protein syntheses.

7. Total chemical synthesis of SUMO-2-Lys63-linked diubiquitin hybrid chains assisted by removable solubilizing tags.

8. Effect of Methionine Sulfoxide on the Synthesis and Purification of Aggregation-Prone Peptides.

9. Chemical synthesis of membrane proteins: a model study on the influenza virus B proton channel.

10. Palladium prompted on-demand cysteine chemistry for the synthesis of challenging and uniquely modified proteins.