Literature DB >> 27265428

A gradient-free method for the purification of infective dengue virus for protein-level investigations.

Stephanie M Jensen1, Celina T Nguyen1, John C Jewett2.   

Abstract

Dengue virus (DENV) is a mosquito-transmitted flavivirus that infects approximately 100 million people annually. Multi-day protocols for purification of DENV reduce the infective titer due to viral sensitivity to both temperature and pH. Herein we describe a 5-h protocol for the purification of all DENV serotypes, utilizing traditional gradient-free ultracentrifugation followed by selective virion precipitation. This protocol allows for the separation of DENV from contaminating proteins - including intact C6/36 densovirus, for the production of infective virus at high concentration for protein-level analysis.
Copyright © 2016 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Aedes albopictus; Dengue virus; Densovirus; Infectivity; Precipitation; Purification

Mesh：

Year:  2016        PMID： 27265428      PMCID： PMC4992633          DOI： 10.1016/j.jviromet.2016.05.017

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  23 in total

1.  Growth and production of the dengue virus in C6/36 cells and identification of a laminin-binding protein as a candidate serotype 3 and 4 receptor protein.

Authors:  Prirayapak Sakoonwatanyoo; Verawan Boonsanay; Duncan R Smith
Journal:  Intervirology       Date:  2006       Impact factor: 1.763

2.  Putative dengue virus receptors from mosquito cells.

Authors:  M L Muñoz; A Cisneros; J Cruz; P Das; R Tovar; A Ortega
Journal:  FEMS Microbiol Lett       Date:  1998-11-15       Impact factor: 2.742

3.  Structural changes in dengue virus when exposed to a temperature of 37°C.

Authors:  Guntur Fibriansah; Thiam-Seng Ng; Victor A Kostyuchenko; Jaime Lee; Sumarlin Lee; Jiaqi Wang; Shee-Mei Lok
Journal:  J Virol       Date:  2013-05-01       Impact factor: 5.103

4.  Genetic, biochemical, and structural characterization of a new densovirus isolated from a chronically infected Aedes albopictus C6/36 cell line.

Authors:  Senxiong Chen; Lingpeng Cheng; Qinfen Zhang; Wei Lin; Xinying Lu; Jennifer Brannan; Z H Zhou; Jingqiang Zhang
Journal:  Virology       Date:  2004-01-05       Impact factor: 3.616

5.  Isolation of a Singh's Aedes albopictus cell clone sensitive to Dengue and Chikungunya viruses.

Authors:  A Igarashi
Journal:  J Gen Virol       Date:  1978-09       Impact factor: 3.891

6.  Structure of the dengue virus envelope protein after membrane fusion.

Authors:  Yorgo Modis; Steven Ogata; David Clements; Stephen C Harrison
Journal:  Nature       Date:  2004-01-22       Impact factor: 49.962

7.  Potassium tartrate-glycerol as a density gradient substrate for separation of small, round viruses from human feces.

Authors:  C R Ashley; E O Caul
Journal:  J Clin Microbiol       Date:  1982-08       Impact factor: 5.948

8.  Dengue virus: isolation, propagation, quantification, and storage.

Authors:  Freddy Medina; Juan Francisco Medina; Candimar Colón; Edgardo Vergne; Gilberto A Santiago; Jorge L Muñoz-Jordán
Journal:  Curr Protoc Microbiol       Date:  2012-11

9.  A toggle switch controls the low pH-triggered rearrangement and maturation of the dengue virus envelope proteins.

Authors:  Aihua Zheng; Fei Yuan; Lara M Kleinfelter; Margaret Kielian
Journal:  Nat Commun       Date:  2014-05-20       Impact factor: 14.919

10.  Zika virus in Gabon (Central Africa)--2007: a new threat from Aedes albopictus?

Authors:  Gilda Grard; Mélanie Caron; Illich Manfred Mombo; Dieudonné Nkoghe; Statiana Mboui Ondo; Davy Jiolle; Didier Fontenille; Christophe Paupy; Eric Maurice Leroy
Journal:  PLoS Negl Trop Dis       Date:  2014-02-06
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  2 in total

1.  Light-Activated Triazabutadienes for the Modification of a Viral Surface.

Authors:  Stephanie M Jensen; Flora W Kimani; John C Jewett
Journal:  Chembiochem       Date:  2016-10-21       Impact factor: 3.164

2.  Development of a Rapid Gold Nanoparticle-Based Lateral Flow Immunoassay for the Detection of Dengue Virus.

Authors:  Cynthia Martinez-Liu; Carlos Machain-Williams; Natalia Martinez-Acuña; Sonia Lozano-Sepulveda; Kame Galan-Huerta; Daniel Arellanos-Soto; Mayra Meléndez-Villanueva; Diana Ávalos-Nolazco; Katya Pérez-Ibarra; Sergio Galindo-Rodríguez; Aurora de Jesús Garza-Juarez; Ana María Rivas-Estilla
Journal:  Biosensors (Basel)       Date:  2022-07-07
  2 in total

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