| Literature DB >> 27265372 |
A V Ibarra-Meneses1, E Carrillo2, C Sánchez1, J García-Martínez3, D López Lacomba3, J V San Martin4, F Alves5, J Alvar5, J Moreno1.
Abstract
No field method exists for identifying asymptomatic individuals in areas where Leishmania infantum is endemic. This work reports that, 24 h after stimulating whole blood with soluble Leishmania antigen (SLA), plasma interferon-γ (IFN-γ) and interleukin-2 (IL-2) become significantly elevated in samples from asymptomatic individuals (n=47) compared with those from negative controls (n=50), all of them recruited from a blood bank. When compared with the reference test SLA-lymphoproliferative assay, IL-2 appears as a new, 100% sensitive and specific marker for asymptomatic individuals with a positive cellular response (compared with 100% and 84.78%, respectively, for IFN-γ). Further studies in other transmission areas and in other cohorts of exposed people need to be performed to confirm these results. Once validated, IFN-γ and IL-2 levels in SLA-stimulated whole blood could be reliably used in the field to estimate the prevalence of those asymptomatic individuals with Leishmania-specific cellular immune responses.Entities:
Keywords: Asymptomatic; Interferon-γ release assay; Interleukin-2; Leishmaniasis; Marker
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Year: 2016 PMID： 27265372 DOI： 10.1016/j.cmi.2016.05.021
Source DB: PubMed Journal: Clin Microbiol Infect ISSN： 1198-743X Impact factor: 8.067