Ivana Musilova1, Ctirad Andrys2, Marcela Drahosova2, Ondrej Soucek2, Martin Stepan1, Tomas Bestvina1, Richard Spacek3, Bo Jacobsson4,5, Teresa Cobo4,6,7, Marian Kacerovsky1,8. 1. a Department of Obstetrics and Gynecology , Faculty of Medicine, Charles University in Prague, Hradec Kralove, University Hospital Hradec Kralove , Hradec Kralove , Czech Republic. 2. b Department of Clinical Immunology and Allergy , Faculty of Medicine, Charles University in Prague, Hradec Kralove, University Hospital Hradec Kralove , Hradec Kralove , Czech Republic. 3. c Department of Obstetrics and Gynecology , Faculty of Medicine, Ostrava University , Ostrava , Czech Republic. 4. d Department of Obstetrics and Gynecology , Sahlgrenska Academy, Gothenburg University , Gothenburg , Sweden. 5. e Department of Genetics and Bioinformatics Area of Health Data and Digitalisation , Institute of Public Health , Oslo , Norway. 6. f BCNatal-Barcelona Center for Maternal-Fetal and Neonatal Medicine (Hospital Clínic and Hospital Sant Joan De Deu), Fetal I + D Fetal Medicine Research Center, IDIBAPS, University of Barcelona , Barcelona , Spain. 7. g Center for Biomedical Research on Rare Diseases (CIBER-ER) , Barcelona , Spain , and. 8. h Biomedical Research Center, University Hospital Hradec Kralove , Hradec Kralove , Czech Republic.
Abstract
OBJECTIVE: To evaluate umbilical cord blood interleukin (IL)-6 concentrations and the occurrence of fetal inflammatory response syndrome (FIRS) with respect to microbial invasion of the amniotic cavity (MIAC) and/or intraamniotic inflammation (IAI) in pregnancies complicated by preterm prelabor rupture of membranes (PPROM). METHODS: One-hundred-eighty-eight women with singleton pregnancies complicated by PPROM between gestational ages of 24 + 0 and 36 + 6 weeks were included in the study. Blood samples were obtained by venipuncture from the umbilical cord after the delivery of the newborn. The umbilical cord blood IL-6 concentrations were evaluated using ELISA kits. FIRS was defined as umbilical cord blood IL-6 > 11 pg/mL. RESULT: Women with MIAC and IAI had higher IL-6 concentrations than women without these complications (with MIAC: median 18.1 pg/mL versus without MIAC: median 5.8; p < 0.0001; with IAI: median 32.9 pg/mL, versus without IAI: median 5.8; p < 0.0001). Women with IAI with MIAC and women with IAI without MIAC had the highest umbilical cord blood IL-6 concentrations (medians: 32.6 and 39.4 pg/mL) and rates of FIRS (78% and 67%). CONCLUSIONS: IAI was associated with the highest umbilical cord blood IL-6 concentrations and rate of FIRS independent of the presence or absence of MIAC.
OBJECTIVE: To evaluate umbilical cord blood interleukin (IL)-6 concentrations and the occurrence of fetal inflammatory response syndrome (FIRS) with respect to microbial invasion of the amniotic cavity (MIAC) and/or intraamniotic inflammation (IAI) in pregnancies complicated by preterm prelabor rupture of membranes (PPROM). METHODS: One-hundred-eighty-eight women with singleton pregnancies complicated by PPROM between gestational ages of 24 + 0 and 36 + 6 weeks were included in the study. Blood samples were obtained by venipuncture from the umbilical cord after the delivery of the newborn. The umbilical cord blood IL-6 concentrations were evaluated using ELISA kits. FIRS was defined as umbilical cord blood IL-6 > 11 pg/mL. RESULT: Women with MIAC and IAI had higher IL-6 concentrations than women without these complications (with MIAC: median 18.1 pg/mL versus without MIAC: median 5.8; p < 0.0001; with IAI: median 32.9 pg/mL, versus without IAI: median 5.8; p < 0.0001). Women with IAI with MIAC and women with IAI without MIAC had the highest umbilical cord blood IL-6 concentrations (medians: 32.6 and 39.4 pg/mL) and rates of FIRS (78% and 67%). CONCLUSIONS: IAI was associated with the highest umbilical cord blood IL-6 concentrations and rate of FIRS independent of the presence or absence of MIAC.
Authors: Ivana Musilova; Ctirad Andrys; Marcela Drahosova; Barbora Zednikova; Helena Hornychova; Lenka Pliskova; Helena Zemlickova; Bo Jacobsson; Marian Kacerovsky Journal: Pediatr Res Date: 2017-12-20 Impact factor: 3.756
Authors: Ivana Musilova; Lenka Pliskova; Romana Gerychova; Petr Janku; Ondrej Simetka; Petr Matlak; Bo Jacobsson; Marian Kacerovsky Journal: PLoS One Date: 2017-12-12 Impact factor: 3.240
Authors: Ivana Musilova; Marian Kacerovsky; Martin Stepan; Tomas Bestvina; Lenka Pliskova; Barbora Zednikova; Bo Jacobsson Journal: PLoS One Date: 2017-08-16 Impact factor: 3.240
Authors: Gregory P Goldstein; Stephanie A Leonard; Peiyi Kan; Euna B Koo; Henry C Lee; Suzan L Carmichael Journal: J Perinatol Date: 2019-04-01 Impact factor: 2.521
Authors: Hassendrini N Peiris; Roberto Romero; Kanchan Vaswani; Sarah Reed; Nardhy Gomez-Lopez; Adi L Tarca; Dereje W Gudicha; Offer Erez; Eli Maymon; Murray D Mitchell Journal: J Matern Fetal Neonatal Med Date: 2019-12-29