Literature DB >> 27265124

Maintenance of High Cytochrome P450 Expression in HepaRG Cell Spheroids in DMSO-Free Medium.

A V Aleksandrova1, O A Burmistrova2, K A Fomicheva3, D A Sakharov2,4.   

Abstract

We studied the effects of DMSO and fibroblasts during HepaRG cell spheroid formation and conditions of their subsequent culturing on the levels of mRNA of the major cytochromes P450. A protocol of spheroid formation from differentiated HepaRG cells and their culturing in serum- and DMSO-free medium is developed.

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Keywords:  HepaRG; cytochromes P450; differentiation

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Year:  2016        PMID: 27265124     DOI: 10.1007/s10517-016-3360-z

Source DB:  PubMed          Journal:  Bull Exp Biol Med        ISSN: 0007-4888            Impact factor:   0.804


  1 in total

1.  DMSO-free highly differentiated HepaRG spheroids for chronic toxicity, liver functions and genotoxicity studies.

Authors:  Marie Cuvellier; Frédéric Ezan; Georges Baffet; Sophie Langouët; Sophie Rose; Jennifer Carteret; Arnaud Bruyère; Vincent Legagneux; Fabrice Nesslany
Journal:  Arch Toxicol       Date:  2021-11-11       Impact factor: 5.153

  1 in total

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