Literature DB >> 27263001

Agrobacterium tumefaciens-mediated transformation of Botryosphaeria dothidea.

Liang Chen1,2, Qun Wang3, Hua Chen4, Gengwu Sun1, Huixiang Liu5, Hongkai Wang6.   

Abstract

Botryosphaeria dothidea is a severe causal agent of die-back and cankers of many woody plants and causes great losses in many regions. The pathogenic mechanism of this pathogen has not been well explored due to lack of mutants and genetic information. In this study, we developed an Agrobacterium tumefaciens-mediated transformation (ATMT) protocol for B. dothidea protoplasts using vector pBHt2 containing the hph gene as a selection marker under the control of trp C promoter. Using this protocol we successfully generated the B. dothidea transformants with efficiency about 23 transformants per 10(5) protoplasts. This is the first report of genetic transformation of B. dothidea via ATMT and this protocol provides an effective tool for B. dothidea genome manipulation, gene identification and functional analysis.

Entities:  

Keywords:  ATMT; Botryosphaeria dothidea; Hygromycin B; T-DNA; hph

Mesh：

Substances：

Year:  2016        PMID： 27263001     DOI： 10.1007/s11274-016-2045-0

Source DB:  PubMed          Journal:  World J Microbiol Biotechnol        ISSN： 0959-3993            Impact factor:   3.312


  12 in total

1.  Agrobacterium tumefaciens-mediated transformation of the plant pathogenic fungus, Magnaporthe grisea.

Authors:  H S Rho; S Kang; Y H Lee
Journal:  Mol Cells       Date:  2001-12-31       Impact factor: 5.034

2.  [Transformation system of Aspergillus japonicus mediated by Agrobacterium tumefaciens].

Authors:  Hui Guo; Zhe Yang; Laijun Xing; Mingchun Li
Journal:  Wei Sheng Wu Xue Bao       Date:  2011-01

3.  Genetically transformed maize plants from protoplasts.

Authors:  C A Rhodes; D A Pierce; I J Mettler; D Mascarenhas; J J Detmer
Journal:  Science       Date:  1988-04-08       Impact factor: 47.728

4.  Morphology and phylogeny of Botryosphaeria dothidea causing fruit rot of olives.

Authors:  A J L Phillips; I C Rumbos; A Alves; A Correia
Journal:  Mycopathologia       Date:  2005-04       Impact factor: 2.574

Review 5.  Agrobacterium-mediated transformation as a tool for functional genomics in fungi.

Authors:  Caroline B Michielse; Paul J J Hooykaas; Cees A M J J van den Hondel; Arthur F J Ram
Journal:  Curr Genet       Date:  2005-05-12       Impact factor: 3.886

6.  Peroxisomal metabolic function is required for appressorium-mediated plant infection by Colletotrichum lagenarium.

Authors:  A Kimura; Y Takano; I Furusawa; T Okuno
Journal:  Plant Cell       Date:  2001-08       Impact factor: 11.277

7.  AAL-Toxin-Deficient Mutants of Alternaria alternata Tomato Pathotype by Restriction Enzyme-Mediated Integration.

Authors:  H Akamatsu; Y Itoh; M Kodama; H Otani; K Kohmoto
Journal:  Phytopathology       Date:  1997-09       Impact factor: 4.025

8.  Agrobacterium tumefaciens-mediated transformation of filamentous fungi.

Authors:  M J de Groot; P Bundock; P J Hooykaas; A G Beijersbergen
Journal:  Nat Biotechnol       Date:  1998-09       Impact factor: 54.908

9.  Agrobacterium tumefaciens-mediated transformation for investigating pathogenicity genes of the phytopathogenic fungus Colletotrichum sansevieriae.

Authors:  Masayuki Nakamura; Hideto Kuwahara; Keisuke Onoyama; Hisashi Iwai
Journal:  Curr Microbiol       Date:  2012-05-15       Impact factor: 2.188

10.  Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae.

Authors:  P Bundock; A den Dulk-Ras; A Beijersbergen; P J Hooykaas
Journal:  EMBO J       Date:  1995-07-03       Impact factor: 11.598
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  1 in total

1.  ATMT transformation efficiencies with native promoters in Botryosphaeria kuwatsukai causing ring rot disease in pear.

Authors:  Xueying Gu; Jiamin Zhao; Hongkai Wang; Fu-Cheng Lin; Qingyuan Guo; Neeraj Shrivastava; Rajesh Jeewon
Journal:  World J Microbiol Biotechnol       Date:  2018-11-19       Impact factor: 3.312
  1 in total

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