Literature DB >> 27262188

Overview of Fusion Tags for Recombinant Proteins.

E N Kosobokova1, K A Skrypnik, V S Kosorukov.   

Abstract

Virtually all recombinant proteins are now prepared using fusion domains also known as "tags". The use of tags helps to solve some serious problems: to simplify procedures of protein isolation, to increase expression and solubility of the desired protein, to simplify protein refolding and increase its efficiency, and to prevent proteolysis. In this review, advantages and disadvantages of such fusion tags are analyzed and data on both well-known and new tags are generalized. The authors own data are also presented.

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Year:  2016        PMID： 27262188     DOI： 10.1134/S0006297916030019

Source DB:  PubMed          Journal:  Biochemistry (Mosc)        ISSN： 0006-2979            Impact factor:   2.487


  15 in total

1.  Nicotinamide phosphoribosyltransferase purification using SUMO expression system.

Authors:  Trivikram R Molugu; Radu C Oita; Udeep Chawla; Sara M Camp; Michael F Brown; Joe G N Garcia
Journal:  Anal Biochem       Date:  2020-01-23       Impact factor: 3.365

Review 2.  A Review: Molecular Chaperone-mediated Folding, Unfolding and Disaggregation of Expressed Recombinant Proteins.

Authors:  Komal Fatima; Fatima Naqvi; Hooria Younas
Journal:  Cell Biochem Biophys       Date:  2021-02-25       Impact factor: 2.194

3.  Advances and Insights for Small Molecule Inhibition of Macrophage Migration Inhibitory Factor.

Authors:  Vinay Trivedi-Parmar; William L Jorgensen
Journal:  J Med Chem       Date:  2018-06-04       Impact factor: 7.446

4.  Enhanced production of taxadiene in Saccharomyces cerevisiae.

Authors:  Behnaz Nowrouzi; Rachel A Li; Laura E Walls; Leo d'Espaux; Koray Malcı; Lungang Liang; Nestor Jonguitud-Borrego; Albert I Lerma-Escalera; Jose R Morones-Ramirez; Jay D Keasling; Leonardo Rios-Solis
Journal:  Microb Cell Fact       Date:  2020-11-02       Impact factor: 5.328

5.  Comparison of Periplasmic and Cytoplasmic Expression of Bovine Enterokinase Light Chain in E. coli.

Authors:  Hoda Ayat; Omid Darvishi; Elham Moazeni; Asiye Momeni Bidezard
Journal:  Protein J       Date:  2022-01-29       Impact factor: 2.371

6.  Purification of recombinant human chemokine CCL2 in E. coli and its function in ovarian cancer.

Authors:  Wei Liu; Lei Wang; Jiajia Zhang; Liuhui Qiao; Yiqing Liu; Xinke Yang; Jinghua Zhang; Wenming Zheng; Zhenling Ma
Journal:  3 Biotech       Date:  2021-01-02       Impact factor: 2.406

7.  Synechocystis PCC 6803 overexpressing RuBisCO grow faster with increased photosynthesis.

Authors:  Feiyan Liang; Peter Lindblad
Journal:  Metab Eng Commun       Date:  2017-02-20

8.  Single-Input Regulatory Cascade for in vivo Removal of the Solubility Tag in Fusion Recombinant Proteins Produced by Escherichia coli.

Authors:  Filipe S R Silva; Sara P O Santos; Roberto Meyer; Neuza M Alcantara-Neves; Carina S Pinheiro; Luis G C Pacheco
Journal:  Front Bioeng Biotechnol       Date:  2019-08-20

Review 9.  Antifungal Peptides as Therapeutic Agents.

Authors:  Miguel Fernández de Ullivarri; Sara Arbulu; Enriqueta Garcia-Gutierrez; Paul D Cotter
Journal:  Front Cell Infect Microbiol       Date:  2020-03-17       Impact factor: 5.293

10.  Improved Plasmid-Based Inducible and Constitutive Gene Expression in Corynebacterium glutamicum.

Authors:  Nadja A Henke; Irene Krahn; Volker F Wendisch
Journal:  Microorganisms       Date:  2021-01-19
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