Marta Giovanetti1, Teresa Milano2, Luiz Carlos Alcantara3, Laura Carcangiu4, Eleonora Cella5, Alessia Lai6, Alessandra Lo Presti4, Stefano Pascarella2, Gianguglielmo Zehender6, Silvia Angeletti7, Massimo Ciccozzi8. 1. Department of Infectious Parasitic and Immunomediated Diseases, National Institute of Health, Rome, Italy; Department of Biology, University of Rome 'Tor Vergata', Rome, Italy; Laboratory of Hematology, Genetic and Computational Biology, Gonçalo Moniz Research Center, Oswaldo Cruz Foundation (LHGB/CPqGM/FIOCRUZ), Salvador, Bahia, Brazil. 2. Dipartimento di Scienze Biochimiche 'A. Rossi Fanelli', Università La Sapienza, 00185 Roma, Italy. 3. Laboratory of Hematology, Genetic and Computational Biology, Gonçalo Moniz Research Center, Oswaldo Cruz Foundation (LHGB/CPqGM/FIOCRUZ), Salvador, Bahia, Brazil. 4. Department of Infectious Parasitic and Immunomediated Diseases, National Institute of Health, Rome, Italy. 5. Department of Infectious Parasitic and Immunomediated Diseases, National Institute of Health, Rome, Italy; Department of Public Health and Infectious Diseases, Sapienza University of Rome, Rome, Italy. 6. Laboratory of Infectious Diseases and Tropical Medicine, University of Milan, Italy. 7. Clinical Pathology and Microbiology Laboratory, University Hospital Campus Bio-Medico of Rome, Rome, Italy. 8. Department of Infectious Parasitic and Immunomediated Diseases, National Institute of Health, Rome, Italy; University Campus Bio-Medico, Rome, Italy. Electronic address: ciccozzi@iss.it.
Abstract
OBJECTIVE: To investigate the genetic diversity of Zika Virus (ZIKV) and the relationships existing among these circulating viruses worldwide. To evaluate the genetic polymorphisms harbored from ZIKV that can have an influence on the virus circulation. METHODS: Three different ZIKV dataset were built. The first dataset included 63 E gene sequences, the second one 22 NS3 sequences and the third dataset was composed of 108 NS5 gene sequences. Phylogenetic and selective pressure analysis was performed. The edited nucleic acid alignment from the Envelope dataset was used to generate a conceptual translation to the corresponding peptide sequences through UGene software. RESULTS: The phylogeographic reconstruction was able to discriminate unambiguously that the Brazilian strains are belonged to the Asian lineage. The structural analysis reveals instead the presence of the Ser residue in the Brazilian sequences (however already observed in other previously reported ZIKV infections) that could suggest the presence of a neutralization-resistant population of viruses. CONCLUSIONS: Phylogenetic, evolutionary and selective pressure analysis contributed to improve the knowledge on the circulation of ZIKV.
OBJECTIVE: To investigate the genetic diversity of Zika Virus (ZIKV) and the relationships existing among these circulating viruses worldwide. To evaluate the genetic polymorphisms harbored from ZIKV that can have an influence on the virus circulation. METHODS: Three different ZIKV dataset were built. The first dataset included 63 E gene sequences, the second one 22 NS3 sequences and the third dataset was composed of 108 NS5 gene sequences. Phylogenetic and selective pressure analysis was performed. The edited nucleic acid alignment from the Envelope dataset was used to generate a conceptual translation to the corresponding peptide sequences through UGene software. RESULTS: The phylogeographic reconstruction was able to discriminate unambiguously that the Brazilian strains are belonged to the Asian lineage. The structural analysis reveals instead the presence of the Ser residue in the Brazilian sequences (however already observed in other previously reported ZIKV infections) that could suggest the presence of a neutralization-resistant population of viruses. CONCLUSIONS: Phylogenetic, evolutionary and selective pressure analysis contributed to improve the knowledge on the circulation of ZIKV.
Authors: Allison Black; Louise H Moncla; Katherine Laiton-Donato; Barney Potter; Lissethe Pardo; Angelica Rico; Catalina Tovar; Diana P Rojas; Ira M Longini; M Elizabeth Halloran; Dioselina Peláez-Carvajal; Juan D Ramírez; Marcela Mercado-Reyes; Trevor Bedford Journal: BMC Infect Dis Date: 2019-11-12 Impact factor: 3.090