Jie Shen1, XinGang Lu2, WangChun Du3, Jun Zhou4, HongFu Qiu2, JingXian Chen5, XiaoHeng Shen5, MingKang Zhong6. 1. Clinical Pharmacy Laboratory, HuaShan Hospital, FuDan University, Shanghai 200040, PR China. 2. Department of Traditional Chinese Medicine, HuaDong Hospital, FuDan University, Shanghai 200040, PR China. 3. Department of Clinical Medicine, Shanghai University of Medicine & Health Sciences, Shanghai 200040, PR China. 4. Department of Gastric, HuaDong Hospital, FuDan University, 200040, PR China. 5. Department of Traditional Chinese Medicine, RuiJin Hospital, Shanghai JiaoTong University School of Medicine, Shanghai 200025, PR China. 6. Clinical Pharmacy Laboratory, HuaShan Hospital, FuDan University, Shanghai 200040, PR China. Electronic address: mingkangzhong@sina.com.
Abstract
AIM: The agent lobetyol, which is isolated from Lobelia chinensis, was previously shown to be cytotoxicity againts several cancer cell lines in published report. Today, we perform a study in vitro and in vivo to analyze its anti-carcinoma effect in MKN45 cells and to explore the molecular mechanism. MAIN METHODS: The growth inhibition of lobetyol on MKN45 cells was analyzed with MTT and flow cytometry. Hoechst 33342 staining and TUNEL cover glass staining were used to provide the visual evidence of apoptosis. Western blotting assay was performed to study the activation or blocking of related signaling pathways. KEY FINDINGS: Lobetyol induce apoptosis and cell cycle arrest in a time- and dose-dependent manner in MKN45 cells in our study. This process is mediated by the MAPK signaling pathways. This study confirmed the cytotoxicity of lobetyol in MKN45 cells and provided an insight into the molecular mechanism, which demonstrates the potential of lobetyol as an anti-tumor agent.
AIM: The agent lobetyol, which is isolated from Lobelia chinensis, was previously shown to be cytotoxicity againts several cancer cell lines in published report. Today, we perform a study in vitro and in vivo to analyze its anti-carcinoma effect in MKN45 cells and to explore the molecular mechanism. MAIN METHODS: The growth inhibition of lobetyol on MKN45 cells was analyzed with MTT and flow cytometry. Hoechst 33342 staining and TUNEL cover glass staining were used to provide the visual evidence of apoptosis. Western blotting assay was performed to study the activation or blocking of related signaling pathways. KEY FINDINGS: Lobetyol induce apoptosis and cell cycle arrest in a time- and dose-dependent manner in MKN45 cells in our study. This process is mediated by the MAPK signaling pathways. This study confirmed the cytotoxicity of lobetyol in MKN45 cells and provided an insight into the molecular mechanism, which demonstrates the potential of lobetyol as an anti-tumor agent.