| Literature DB >> 27259933 |
Paul S Holcomb1, Michael Morehead2, Gianfranco Doretto2, Peter Chen2, Stuart Berg3, Stephen Plaza3, George Spirou4,5,6,7.
Abstract
Connectomics-the study of how neurons wire together in the brain-is at the forefront of modern neuroscience research. However, many connectomics studies are limited by the time and precision needed to correctly segment large volumes of electron microscopy (EM) image data. We present here a semi-automated segmentation pipeline using freely available software that can significantly decrease segmentation time for extracting both nuclei and cell bodies from EM image volumes.Entities:
Keywords: 3D reconstruction; Connectomics; Electron microscopy; Segmentation; ilastik
Mesh：
Year: 2016 PMID： 27259933 PMCID： PMC4978130 DOI： 10.1007/978-1-4939-3615-1_16
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745