| Literature DB >> 27257257 |
Roni H G Wright1, Antonios Lioutas1, Francois Le Dily1, Daniel Soronellas1, Andy Pohl1, Jaume Bonet2, A S Nacht1, Sara Samino3, Jofre Font-Mateu1, Guillermo P Vicent1, Michael Wierer1, Miriam A Trabado4, Constanze Schelhorn5, Carlo Carolis4, Maria J Macias6, Oscar Yanes3, Baldo Oliva2, Miguel Beato7.
Abstract
Key nuclear processes in eukaryotes, including DNA replication, repair, and gene regulation, require extensive chromatin remodeling catalyzed by energy-consuming enzymes. It remains unclear how the ATP demands of such processes are met in response to rapid stimuli. We analyzed this question in the context of the massive gene regulation changes induced by progestins in breast cancer cells and found that ATP is generated in the cell nucleus via the hydrolysis of poly(ADP-ribose) to ADP-ribose. In the presence of pyrophosphate, ADP-ribose is used by the pyrophosphatase NUDIX5 to generate nuclear ATP. The nuclear source of ATP is essential for hormone-induced chromatin remodeling, transcriptional regulation, and cell proliferation.Entities:
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Year: 2016 PMID： 27257257 DOI： 10.1126/science.aad9335
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728