Literature DB >> 2725523

Complementation of a methotrexate uptake defect in Chinese hamster ovary cells by DNA-mediated gene transfer.

T M Underhill1, W F Flintoff.   

Abstract

A methotrexate-resistant Chinese hamster ovary cell line deficient in methotrexate uptake has been complemented to methotrexate sensitivity by transfection with DNA isolated from either wild-type Chinese hamster ovary or human G2 cells. Primary and secondary transfectants regained the ability to take up methotrexate in a manner similar to that of wild-type cells, and in the case of those transfected with human DNA, to contain human-specific DNA sequences. The complementation by DNA-mediated gene transfer of this methotrexate-resistant phenotype provides a basis for the cloning of a gene involved in methotrexate uptake.

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Year:  1989        PMID： 2725523      PMCID： PMC362594          DOI： 10.1128/mcb.9.4.1754-1758.1989

Source DB:  PubMed          Journal:  Mol Cell Biol        ISSN： 0270-7306            Impact factor:   4.272


  31 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

3.  Isolation of a human X chromosome-linked gene essential for progression from G1 to S phase of the cell cycle.

Authors:  T Sekiguchi; M C Yoshida; M Sekiguchi; T Nishimoto
Journal:  Exp Cell Res       Date:  1987-04       Impact factor: 3.905

4.  Molecular cloning and biological characterization of a human gene, ERCC2, that corrects the nucleotide excision repair defect in CHO UV5 cells.

Authors:  C A Weber; E P Salazar; S A Stewart; L H Thompson
Journal:  Mol Cell Biol       Date:  1988-03       Impact factor: 4.272

5.  Isolation of high-molecular-weight DNA from mammalian cells.

Authors:  M Gross-Bellard; P Oudet; P Chambon
Journal:  Eur J Biochem       Date:  1973-07-02

6.  Characterization of the folate-binding proteins associated with the plasma membrane of rat liver.

Authors:  M da Costa; S P Rothenberg
Journal:  Biochim Biophys Acta       Date:  1988-04-22

7.  Carrier-mediated transport of folate compounds in L1210 cells. Initial rate kinetics and extent of duality of entry routes for folic acid and diastereomers of 5-methyltetrahydrohomofolate in the presence of physiological anions.

Authors:  F M Sirotnak; L J Goutas; D M Jacobsen; L S Mines; J R Barrueco; Y Gaumont; R L Kisliuk
Journal:  Biochem Pharmacol       Date:  1987-05-15       Impact factor: 5.858

8.  Transfection of normal human and Chinese hamster DNA corrects diepoxybutane-induced chromosomal hypersensitivity of Fanconi anemia fibroblasts.

Authors:  M Shaham; B Adler; S Ganguly; R S Chaganti
Journal:  Proc Natl Acad Sci U S A       Date:  1987-08       Impact factor: 11.205

9.  Purified membrane and soluble folate binding proteins from cultured KB cells have similar amino acid compositions and molecular weights but differ in fatty acid acylation.

Authors:  C A Luhrs; P Pitiranggon; M da Costa; S P Rothenberg; B L Slomiany; L Brink; G I Tous; S Stein
Journal:  Proc Natl Acad Sci U S A       Date:  1987-09       Impact factor: 11.205

10.  Further studies on a novel class of genetic variants of the L1210 cell with increased folate analogue transport inward. Transport properties of a new variant, evidence for increased levels of a specific transport protein, and its partial characterization following affinity labeling.

Authors:  C H Yang; F M Sirotnak; L S Mines
Journal:  J Biol Chem       Date:  1988-07-15       Impact factor: 5.157
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  2 in total

1.  Stable transfectants of human MCF-7 breast cancer cells with increased levels of the human folate receptor exhibit an increased sensitivity to antifolates.

Authors:  K N Chung; Y Saikawa; T H Paik; K H Dixon; T Mulligan; K H Cowan; P C Elwood
Journal:  J Clin Invest       Date:  1993-04       Impact factor: 14.808

2.  Delineating the extracellular water-accessible surface of the proton-coupled folate transporter.

Authors:  Phaneendra Kumar Duddempudi; Raman Goyal; Swapneeta Sanjay Date; Michaela Jansen
Journal:  PLoS One       Date:  2013-10-18       Impact factor: 3.240
  2 in total

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