Li Li1, Lishou Xiong2, Junhua Yao3, Xiaojun Zhuang1, Shenghong Zhang1, Qiao Yu1, Yinglian Xiao1, Yi Cui1, Minhu Chen1. 1. Department of Gastroenterology and Hepatology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China. 2. Department of Gastroenterology and Hepatology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China. Electronic address: xionglishou@263.net. 3. Instrumental Analysis and Research Centre, Sun Yat-Sen University, Guangzhou, China.
Abstract
BACKGROUND: Altered intestinal permeability in diarrhoea-predominant irritable bowel syndrome (IBS-D) has been reported in some studies. AIMS: The study aimed to investigate the altered intestinal permeability and its associated clinical characteristics and RNA expression profiles in IBS-D. METHODS: We stratified IBS-D patients into two groups according to the P95 value of the permeability in controls. The clinical characteristics of the two groups were evaluated, and two biopsy cases from each of the two groups were selected for the RNA-seq analysis. RESULTS: IBS-D patients had a significant increase in the small intestinal permeability compared with controls [0.0245 (0.0229) median (interquartile range)] versus 0.0156 (0.0098), P=0.010), but no significant difference was found in the colonic permeability [23.286 (10.470) versus 21.650 (6.650), P=0.574]. The IBS-D patients with increased small intestinal permeability had worse psychological effects (P=0.027) and quality of life (P=0.044). Analysis of RNA-seq data revealed 185 genes differentially expressed, many of which were related to mucosal inflammation and immunity. CONCLUSIONS: Small intestinal permeability, but not colonic permeability, is increased in IBS-D patients. IBS-D patients with increased small intestinal permeability tend to be more severely impaired in terms of psychological effects and quality of life, and analysis of RNA-seq data reveals that increased small intestinal permeability is related to mucosal inflammation and immunity.
BACKGROUND: Altered intestinal permeability in diarrhoea-predominant irritable bowel syndrome (IBS-D) has been reported in some studies. AIMS: The study aimed to investigate the altered intestinal permeability and its associated clinical characteristics and RNA expression profiles in IBS-D. METHODS: We stratified IBS-D patients into two groups according to the P95 value of the permeability in controls. The clinical characteristics of the two groups were evaluated, and two biopsy cases from each of the two groups were selected for the RNA-seq analysis. RESULTS: IBS-D patients had a significant increase in the small intestinal permeability compared with controls [0.0245 (0.0229) median (interquartile range)] versus 0.0156 (0.0098), P=0.010), but no significant difference was found in the colonic permeability [23.286 (10.470) versus 21.650 (6.650), P=0.574]. The IBS-D patients with increased small intestinal permeability had worse psychological effects (P=0.027) and quality of life (P=0.044). Analysis of RNA-seq data revealed 185 genes differentially expressed, many of which were related to mucosal inflammation and immunity. CONCLUSIONS: Small intestinal permeability, but not colonic permeability, is increased in IBS-D patients. IBS-D patients with increased small intestinal permeability tend to be more severely impaired in terms of psychological effects and quality of life, and analysis of RNA-seq data reveals that increased small intestinal permeability is related to mucosal inflammation and immunity.
Authors: Nikita Hanning; Adam L Edwinson; Hannah Ceuleers; Stephanie A Peters; Joris G De Man; Leslie C Hassett; Benedicte Y De Winter; Madhusudan Grover Journal: Therap Adv Gastroenterol Date: 2021-02-24 Impact factor: 4.409
Authors: Bruno K Rodiño-Janeiro; Cristina Martínez; Marina Fortea; Beatriz Lobo; Marc Pigrau; Adoración Nieto; Ana María González-Castro; Eloísa Salvo-Romero; Danila Guagnozzi; Cristina Pardo-Camacho; Cristina Iribarren; Fernando Azpiroz; Carmen Alonso-Cotoner; Javier Santos; Maria Vicario Journal: Sci Rep Date: 2018-02-02 Impact factor: 4.379