| Literature DB >> 27246735 |
Chao Dong1, Leilei Niu1, Wei Song1, Xin Xiong2, Xianhua Zhang2, Zhenxi Zhang1, Yi Yang1, Fan Yi1, Jun Zhan3, Hongquan Zhang3, Zhenjun Yang1, Li-He Zhang1, Suodi Zhai2, Hua Li4, Min Ye5, Quan Du6.
Abstract
Despite the recent progress in RNA modification study, a comprehensive modification profile is still lacking for mammalian cells. Using a quantitative HPLC/MS/MS assay, we present here a study where RNA modifications are examined in term of the major RNA species. With paired slow- and fast-proliferating cell lines, distinct RNA modification profiles are first revealed for diverse RNA species. Compared to mRNAs, increased ribose and nucleobase modifications are shown for the highly-structured tRNAs and rRNAs, lending support to their contribution to the formation of high-order structures. This study also reveals a dynamic tRNA modification profile in the fast-proliferating cells. In addition to cultured cells, this unique tRNA profile has been further confirmed with endometrial cancers and their adjacent normal tissues. Taken together, the results indicate that tRNA is a actively regulated RNA species in the fast-proliferating cancer cells, and suggest that they may play a more active role in biological process than expected.Entities:
Keywords: Cancer; Proliferation; RNA modification
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Year: 2016 PMID： 27246735 DOI： 10.1016/j.bbrc.2016.05.124
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575