| Literature DB >> 27246213 |
John Powers1,2, Maritza Lienlaf1, Patricio Perez-Villarroel1, Susan Deng1, Tessa Knox1, Alejandro Villagra1, Eva Sahakian3,4.
Abstract
Histone deacetylase 10 (HDAC10) belongs to the class IIb HDAC family and its biological role remains mostly unidentified. A decreased HDAC10 expression has been reported in patients with aggressive solid tumors (Osada et al. Int J Cancer 112: 26-32, 2004; Jin et al. Int J Clin Exp Pathol 7: 5872-5879, 2014), suggesting that loss of HDAC10 expression might confer a survival advantage to malignant cells. Consequently, results from our lab suggests that overexpression of HDAC10 in aggressive mantle cell lymphoma (MCL) and chronic lymphocytic leukemia (CLL) Z138c and MEC1 cells, respectively, resulted in a rapid induction of cell death in vitro with only 5 % of cells being alive at 48 h, cell cycle arrest, and up-regulation of co-stimulatory molecules. Here we present several standard methods to study the function of HDAC10 in B cell malignancies.Entities:
Keywords: Adenoviral overexpression; Genotyping; High-throughput flow screening; Histone deacetylase 10; PCR; Western blotting; qRT-PCR
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Year: 2016 PMID： 27246213 DOI： 10.1007/978-1-4939-3667-0_10
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745