| Literature DB >> 27246210 |
Junyao Yang1, Andriana Margariti2, Lingfang Zeng3.
Abstract
Histone deacetylases (HDACs) have a central role in the regulation of gene expression, which undergoes alternative splicing during embryonic stem cell (ES) cell differentiation. Alternative splicing gives rise to vast diversity over gene information, arousing public concerns in the last decade. In this chapter, we describe a strategy to detect HDAC7 alternative splicing and analyze its function on ES cell differentiation.Entities:
Keywords: Alternative splicing; HDAC7; Luciferase assay; Plasmid construction
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Year: 2016 PMID： 27246210 DOI： 10.1007/978-1-4939-3667-0_7
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745