| Literature DB >> 27245345 |
Mutya Cruz1, Miao Wang1, Jessica Frisch-Daiello1, Xianlin Han2.
Abstract
Extraction of lipids from biological samples is a critical step in lipidomics, especially for shotgun lipidomics where lipid extracts are directly infused into a mass spectrometer. The butanol-methanol (BUME) extraction method was originally developed to extract lipids from plasma samples with 1 % acetic acid. Considering some lipids are sensitive to acidic environments, we modified this protocol by replacing acetic acid with lithium chloride solution and extended the modified extraction to tissue samples. Although no significant reduction of plasmalogen levels in the acidic BUME extracts of rat heart samples was found, the modified method was established to extract various tissue samples, including rat liver, heart, and plasma. Essentially identical profiles of the majority of lipid classes were obtained from the extracts of the modified BUME and traditional Bligh-Dyer methods. However, it was found that neither the original, nor the modified BUME method was suitable for 4-hydroxyalkenal species measurement in biological samples.Entities:
Keywords: BUME; Lipid extraction; Lipidomics; Mass spectrometry; Plasmalogens; Shotgun lipidomics
Mesh：
Substances：
Year: 2016 PMID： 27245345 PMCID： PMC4904733 DOI： 10.1007/s11745-016-4164-7
Source DB: PubMed Journal: Lipids ISSN： 0024-4201 Impact factor: 1.880