| Literature DB >> 27243475 |
Sandip V Mulay1,2, Minsuk Choi3, Yoon Jeong Jang2, Youngsam Kim1,2, Sangyong Jon3, David G Churchill4,5.
Abstract
Two closely related phenyl selenyl based boron-dipyrromethene (BODIPY) turn-on fluorescent probes for the detection of hypochlorous acid (HOCl) were synthesized for studies in chemical biology; emission intensity is modulated by a photoinduced electron-transfer (PET) process. Probe 2 intrinsically shows a negligible background signal; however, after reaction with HOCl, chemical oxidation of selenium forecloses the PET process, which evokes a significant increase in fluorescence intensity. The fluorescence intensity of probes 1 and 2 with HOCl involves an ∼18 and ∼50-fold enhancement compared with the respective responses from other reactive oxygen/nitrogen species (ROS/RNS) and low detection limits (30.9 nm for 1 and 4.5 nm for 2). Both probes show a very fast response with HOCl; emission intensity reached a maximum within 1 s. These probes show high selectivity for HOCl, as confirmed by confocal microscopy imaging when testing with RAW264.7 and MCF-7 cells.Entities:
Keywords: BODIPY; biosensing; fluorescence; hypochlorous acid; photoinduced electron transfer
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Year: 2016 PMID： 27243475 DOI： 10.1002/chem.201601270
Source DB: PubMed Journal: Chemistry ISSN： 0947-6539 Impact factor: 5.236